摘要
以不同性别类型的黄瓜高代自交系为材料,根据GenBank中提供的乙烯合成、信号转导有关基因序列、花器官发育相关的黄瓜性别决定蛋白基因、AGMOUS同源异型基因、与性别表达相关的RAPD和ISSR引物,采用Primer Premier 5.00专业引物设计软件,设计可能与性别表达有关特异引物.结果表明,ACS1号引物对(ACS-F1,5′-ATTCAGATGGGT-3′;ACCS-R1,5′-GCCAAAGCTCGACAT-3′),CsACS1G-SCAR引物对(ACS-F1,5′-TTAACTACTTCGGACGGGCATAG-3′;ACCS-R1,5′-AGGTGTTCAGCAAA CATAGGGTG-3′)及RAPD引物S12对不同性别类型的黄瓜基因组DNA的PCR扩增产物表现出明显的多态性.用Mapmaker/Exp 3.0进行连锁分析,发现雌性F基因和ACS_280,CsACS1G-SCAR_430均连锁群,遗传距离分别为31.4,10.0 cM,与S12_280标记不在一个连锁群上.
Genome DNA of different sexual cucumber inbreed lines was amplified by several RAPD, ISSR primers and sequence-specific primers which were designated by Primer Premier 5.00 from DNA sequence of ethylene biosynthesis and ethylene signal transduction pathway, sex determining protein, AGMOUS homologs with the identity of sex organ. The results indicated that two sequence-specific pair primers（ACR pair primers： ACS-F1, 5＇- ATTCAGATGGGT-3＇： ACS-RI, 5＇-GCCAAAGCTCGACAT-3＇; CsACS1G-SCAR primers： ACS-F1, 5＇-TTAA CTACTTCGGACGGGCATAG-3＇; ACS-R~, 5＇-AGGTGTTCAGCAAACATAGGGTG-3＇）and S12 RAPD primer have polymorphisms which were related to sex expression. Mapmaker/Exp 3.0 was used to study the linkage between makers and F gene. Through linkage analysis, F gene was found to be linked with ACS_280, CsACS1G-SCAR_430 whose distances were 31.4, 10 cM respectively.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第4期403-408,共6页
Journal of Hunan Agricultural University(Natural Sciences)
基金
农业部公益性行业(农业)科研专项(nyhyzx07-007)
湖南农业大学引进人才项目(03YJ10)
