摘要
目的:探讨蛋白酶体抑制剂MG-132对大鼠C6胶质瘤细胞体外增殖抑制和诱导凋亡作用。方法:分别以不同浓度(10、20和50μmol·L-1)的MG-132培养C6胶质瘤细胞,通过MTT法检测不同培养时间细胞增殖活力,流式细胞术检测细胞凋亡率,HE染色、AO/EB染色以及透射电镜观察细胞形态学变化。结果:MTT法检测显示10μmol·L-1MG-132作用于C6胶质瘤细胞24、48和72h后,细胞增殖A值(0.46±0.03、0.48±0.03和0.45±0.02)均显著低于正常对照组(P<0.01),且随浓度增加其抑制作用逐渐增强;10μmol·L-1MG-132作用C6胶质瘤细胞12h后即可经流式细胞仪检测到明显的凋亡亚二倍体峰,以不同药物浓度(10、20和50μmol·L-1)处理C6胶质瘤细胞12、24和48h后其细胞凋亡率均显著高于正常对照组(P<0.01);形态学检查呈现凋亡细胞的特征。结论:蛋白酶体抑制剂MG-132在体外可显著抑制大鼠C6胶质瘤细胞增殖并诱导其发生凋亡。
Objective To investigate the inhibition of proliferation and induction of apoptosis by proteasome inhibitor MG-132 on C6 glioma cell in vitro. Methods Rat glioma C6 cells were cultured with different concentrations of proteasome inhibitor MG-132 （10, 20 and 50μmol·L^-1 ）. Cell viability was determined by MTT assay at different cultured periods. Flow cytometry was used to detect apoptosis change and HE and AO/EB staining and electronic microscope were used to detect the morphological changes of apoptotic cells. Results Compared with normal control, MG-132 significantly reduced the viability of C6 cells in 10, 20 and 50 /μmol·L^-1 MG-132 groups （P〈0.01）. Moreover, the inhibitory effect was higher on high concentration MG-132 compared with low dose MG- 132. After treatment with 10 μmol·L^-1 MG-132 for 24 h, the apoptosis characteristic C6 cells were detected by AO/EB and HE staining. Apoptotic sub-G was detected by flow cytometry in C6 cells incubated with 10μmol·L^-1 MG-132 for 12 h and displayed a time-dependent manner, the apoptotic percentages in 10, 20 and 50 μmol·L^-1 MG-132 groups were significantly higher than that in control group （P〈0. 01）. Oonclusion Proteasome inhibitor MG-132 can inhibit C6 cell proliferation and induce C6 cell apoptosis.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2008年第1期69-72,171,共5页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅基金资助课题(20050407-3)
