摘要
目的:研究姜黄素(Curcumin)对宫颈癌Caski细胞的增殖及原癌基因(Pleiotrophin,PTN)表达的影响。方法:MTT法检测不同浓度的姜黄素对Caski细胞增殖的影响;免疫细胞化学染色观察经姜黄素处理组和未处理组细胞中PTN表达的差别;免疫印迹Western blotting技术检测不同浓度的姜黄素对Caski细胞中PTN表达的影响。结果:姜黄素能抑制Caski细胞的增殖,并且这种抑制作用呈剂量依赖性。免疫细胞化学结果显示PTN在实验组中的表达显著低于对照组。Western blotting检测结果显示姜黄素抑制PTN的表达,并且对PTN表达的抑制程度依赖于姜黄素的浓度。结论:姜黄素能抑制宫颈癌Caski细胞的增殖及PTN表达,这种对PTN表达的抑制可能是其抗肿瘤作用机制之一。
Objective：To investigate the effect of curcumin on the proliferation and the protein expression of Pleiotrophin（PTN） in cervical cancer Caski cells, Methods：MTF was used to assay the proliferation of Caski cells which were treated with different concentrations of curcumin, The immunocytochemistry was used to assay the distinctive expression of PTN in Caski cells which were treated by curcumin or not. The effects of curcumin with different concentrations on the protein levels of PTN were tested by Western blotting. Results：The proliferation of Caski cells were inhibited by curcumin in a does-dependent manner. Immunocytochemistry assessment showed the expression of PTN was significant down-regnlated after treated with curcumin. Western blotting showed the curcumin could down-regulate the expression of PTN,and this was a does-dependent action. Conclusion：Curcumin could inhibit the proliferation and the PTN expression of Caski cells significantly,which may be one of the molecular mechanisms anticancer of curcumin.
出处
《重庆医科大学学报》
CAS
CSCD
2008年第7期825-827,共3页
Journal of Chongqing Medical University
关键词
姜黄素
多效因子
宫颈癌
Curcumin
Pleiotmphin
Cervical cancer
