摘要
目的:探讨细胞周期素E(Cyclin-E)在早孕小鼠子宫内膜的的表达及其对小鼠胚胎着床数的影响。方法:应用RT-PCR及免疫组织化学的方法检测早孕小鼠子宫内膜细胞Cyclin-E基因和蛋白的表达规律;观察子宫角注射抗Cyclin-E抗体后对小鼠胚胎着床数的影响。结果:妊娠第1～7d小鼠子宫内膜组织均有Cyclin-E表达,自妊娠2d起表达增强,5d表达量达高峰。小鼠妊娠期Cyclin-E在子宫内膜腔上皮、腺上皮及内膜基质中均有表达。单侧子宫抗Cyclin-E抗体注射后双侧胚胎着床数差值较对照组(注射等体积生理盐水)明显增大。结论:在小鼠胚胎着床过程中,Cyclin-E可能参与调节子宫内膜的增殖分化,在子宫内膜蜕膜化及胚胎着床过程发挥作用。
Objective：To investigate the expression rule of Cyclin-E in endometrium during the early pregnancy and its role in blastocyst implantation in mice. Methods：RT-PCR and immunohistochemistry techniques were applied to detect Cyclin-E gene and protein expression in endometrium from pregnant mice on days 1 - 7. In addition Anti-cyclinE antibody was injected into horns of uterus of day 3 pregnant mice to observe the numbers of implanted blastocysts. Results：Expression of Cyclin-E gene and protein in pregnant mice were steady increasing since day 2 and reaching maximal level on day 5 of pregnancy. Injection of Anti-cyclinE antibody could decrease the numbers of implanted blastocyst compared with that of saline-injected group. Conclusion：Cyclin-E were persistently expressed in mouse endometrium during the early pregnancy and might be concerned with proliferation differentiation of endometrium and participates in the regulation process of mouse blastodyst implantation.
出处
《重庆医科大学学报》
CAS
CSCD
2008年第8期954-958,共5页
Journal of Chongqing Medical University
