摘要
目的:探讨曲格列酮对肿瘤坏死因子α(Tumor necrosis factor-α,TNF-α)诱导的人肾小球系膜细胞表达细胞间粘附分子1(Intercellular adhesion molecule-1,ICAM-1)的影响及其可能机制。方法:将培养的系膜细胞分为空白对照组,TNF-α组,曲格列酮对照组,不同浓度曲格列酮干预组,采用MTT法测系膜细胞增殖,RT-PCR检测ICAM-1mRNA的表达,ELISA检测培养上清中ICAM-1蛋白浓度,免疫组化法检测NF-κB的表达。结果:曲格列酮可抑制系膜细胞的增殖,TNF-α组系膜细胞ICAM-1mRNA和蛋白的表达均比空白对照组增加(P<0.05),曲格列酮干预组比TNF-α组明显下降(P<0.05)。TNF-α组诱导的NF-κB的活化可被曲格列酮所抑制(P<0.05)。结论:曲格列酮可通过抑制NF-κB信号传导途径减少TNF-α诱导的系膜细胞ICAM-1的表达。
Objective：To investigate the effects of troglitazone on cell proliferation and expression of intercellular adhesion molecule-1（ICAM-1 ） induced by tumor necrosis factor-α （TNF-α） in human mesangial cells. Methods：Human mesangial cells were cultured in RPMI-1640 media containing TNF-α with or without troglitazone. Cell proliferation was assessed by MTT;the mRNA and protein expression of ICAM-1 were measured by RT-PCR and ELISA;the protein expression of NF-κB was measured by immunohistochemistry. Results：Troglitazone inhibited the proliferation of mesangial cell. The mRNA and protein expression of ICAM-1 of the cell induced by TNF-α increased significantly（P〈0.05）. Troglitazone inhibited the expression compared with TNF-α group（P〈0.05）.In addition,TNF-α induced up-regulation of NF-κ B protein was remarkably reduced by troglitazone（P〈0.05）. Conclusion：Troglitazone can inhibit TNF-α induced proliferation of mesangial cell and decrease the expression of ICAM-1 protein and mRNA through inhibiting NF-κ B activity.
出处
《重庆医科大学学报》
CAS
CSCD
2008年第8期914-917,922,共5页
Journal of Chongqing Medical University
