摘要
背景：既往的动物实验已多次证实了肝复康对肝纤维化组织具有预防及治疗作用。目的：观察中药肝复康不同浓度含药血清对肝星状细胞T6细胞株Ⅰ、Ⅲ型胶原、a-平滑肌肌动蛋白和金属蛋白酶组织抑制因子1基因表达的影响，以及探讨其抗纤维化的作用及分子机制。设计、时间及地点：随机对照动物实验，于2005-07／2006—07在大连医科大学病理生理学教研室完成。材料：清洁级SD大鼠25只，体质量300～350g，用于制备含药血清；鼠肝星状细胞T6细胞株由上海中医药大学肝病研究所徐列明教授惠赠；中药肝复康由柴胡、当归（补血合血）、黄芪（益气）、赤芍、白芍（活血化瘀）、丹参（活血化瘀）等组成。方法：按肝复康高、中、低剂量（8．32，4．16，2．08mL／只）灌胃制备药物血清。实验分为5组，空白对照组：加含体积分数0．1正常对照血清的DMEM；模型对照组：加含体积分数0．1正常对照血清＋60ug／L血小板衍生生长因子的DMEM；肝复康低、中、高剂量组：分别加含体积分数0．1低、中、高剂量药物血清＋60ug／L血小板衍生生长因子的DMEM；各组均培养24h。主要观察指标：反转录-聚合酶链反应检测不同浓度肝复康药物血清干预后Ⅰ、Ⅲ型胶原、a-平滑肌肌动蛋白和金属蛋白酶组织抑制因子1mRNA的表达。结果：空白对照组肝星状细胞T6细胞各指标表达呈相对低水平，而模型对照组表达明显升高（P〈0．01）；肝复康各剂量组则均具有能明显抑制这种升高的作用（P〈0．01），其中以中剂量组干预效果最为明显。结论：中药肝复康对肝纤维化具有明显的抑制作用，其作用机制可能是通过抑制Ⅰ、Ⅲ型胶原、a-平滑肌肌动蛋白及金属蛋白酶组织抑制因子1mRNA的转录，从而减少细胞外基质的形成，以达到抗纤维化的作用。
BACKGROUND： Previous researches have revealed that Ganfukang compound may prevent and cure hepatic fibrosis. OBJECTIVE： To study the effect of blood serum supplemented with Ganfukang compound, a traditional Chinese herb medicine, on the expression of type Ⅰ,Ⅲ collagen, a -smooth muscle actin （a -SMA） and tissue inhibitor of metalloproteinase-1 （TIMP-1） gene in hepatic stellate cells, and further investigate the anti-fibrosis action and molecule mechanism. DESIGN, TIME AND SETTING： Randomized control animal experiments were carded out in the Department of Pathophysiology, Dalian Medical University （Dalian, Liaoning, China） from July 2005 to July 2006. MATERIALS： Twenty-five SD rats of clean grade, weighing 300-350 g, were used to prepare the drug-containing blood serum; Rat hepatic stellate cells （T6 cell strain） was offered by Professor Xu Lie-ming, Institute of Liver Diseases, Shanghai University of Traditional Chinese Medicine. Ganfukang comprised radix bupleuri, angelica （tonifying blood and oxygenating blood）, radix astragali （supplementing qi）, red peony root, white peony root （promoting blood circulation） and salvia miltiorrhiza （promoting blood circulation）. METHODS： Blood serum was supplemented with Ganfukang compound at high, middle and low concentrations （8.32, 4.16 and 2.08 mL） by lavage. Rat hepatic stellate cells were divided into five groups： blank control group was added with 0.1 mass fraction normal serum; model control group was added with 0.1 mass fraction normal serum and 60 u g/L platelet-derived growth factor; Ganfukang compound groups were added with Ganfukang compound and 60 u g/L platelet-derived growth factor. Each group cells were cultured on DMEM for 24 hours. MAIN OUTCOME MEASURES： The expressions of type Ⅰ and Ⅲ collagen, a -SMA and TIMP-1 among each group were analyzed by reverse transcription-polymerase chain reaction. RESULTS： In the blank control group, hepatic stellate cells exhibited a low level of type Ⅰ and Ⅲ c
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第33期6496-6499,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
高等学校博士学科点专向科研基金(20050161003)~~
