摘要
目的探讨结核菌素对肝癌和肺癌肿瘤细胞生长和凋亡的调控作用。方法制备不同浓度的结核菌上清液(TB-SN),分别与肿瘤细胞株HePG2(肝癌)和A549(肺癌)进行反应。应用特异性荧光探针LIVE/DEAD Viability/Cytotoxicity试剂盒检测肿瘤细胞的生长情况,应用Vybrant凋亡试剂盒检测肿瘤细胞的凋亡情况。结果与5%TB-SN反应5 d后,HePG2和A549细胞凋亡显著增加;与TB-SN反应后,HePG2和A549细胞生长受到抑制。结论结核菌素可以介导肝癌和肺癌肿瘤细胞的凋亡,并抑制肿瘤细胞的生长。
Objective To investigate the regulatory effects of tuberculin on growth and apoptosis of liver cancer and lung cancer cell lines. Methods HePG2（liver cancer） and A549（lung cancer） cell lines were treated with TB supernatant（TB-SN） with different concentrations. Cell viability was detected by using LIVE/DEAD Viability/Cytotoxicity cell kits including specific fluorescence primer,and cell apoptosis was detected by Vybrant apoptosis assay. Results After treatment with 5% TB-SN for 5 d,cell apoptosis was significantly increased in HePG2 and A549 cell lines.Cell growth of HePG2 and A549 cell lines was inhibited after treatment with TB-SN. Conclusion Tuberculin can induce cell apoptosis and inhibit cell growth of liver cancer and lung cancer cell lines.
出处
《上海交通大学学报（医学版）》
CAS
CSCD
北大核心
2008年第8期983-986,共4页
Journal of Shanghai Jiao tong University:Medical Science
