摘要
目的构建含活性真皮基质的组织工程皮肤。方法将人成纤维细胞（Fb）与Ⅰ型牛胶原混合接种于猪脱细胞真皮基质（PADM）的表面，构建活性真皮替代物。其上接种人表皮细胞进行气-液面培养，获得组织工程皮肤，进行组织学观察。结果Fh在胶原内结构完整，与PADM形成复合真皮基质。所构建的组织工程皮肤表皮层结构与人正常皮肤相似，具备基底层、棘层、颗粒层和角质层，细胞之间有桥粒连接，细胞分化良好。结论Fh-胶原-PADM真皮替代物可作为较好的构建组织工程皮肤的真皮支架。
Objective To construct of tissue engineering skin including active composite dermal matrix. Methods The human fibroblasts and bovine collagen with type Ⅰ were inoculated on the surface of porcine acellular dermal matrix（PADM） for construction of active dermal substitute, then epidermal cells were inoculated on the dermal matrix for gas-liquid interface culture. The tissue-engineering skin was observed by histological examinations. Results The structure of fibroblasts in collagen was intact,which was used to construct composite dermal matrix with PADM . The epithelial structure of tissue-engineering skin was similar to that of normal skin with good cell differentiation. Some phenomena were showed in epidermis ： basic layer, stratum spinosum ,granular layer and stratum corneum, desmosomes. Conclusion Fibroblasts-Collagen-PADM can be an optimal dermal matrix for construction of tissue-engineering skin.
出处
《中华烧伤杂志》
CAS
CSCD
北大核心
2008年第4期272-274,共3页
Chinese Journal of Burns
基金
全军医学科学技术研究“十一五”计划专项课题（062054）
首都医学发展科研基金（2005-2040）：北京市科技计划研发攻关课题（H060920050830）
关键词
组织工程
皮肤
人工
细胞培养技术
表皮细胞
真皮基质
Tissue engineering
Skin, artificial
Cell culture techniques
Epithelial cell
Dermal matrix
