摘要
分析安徽道地药材丹皮(Paeonia suffruticosa Andr)及其伪品、混淆品核糖体DNA第二内转录间隔区(ITS2)的序列。分析丹皮的分子遗传多样性,为丹皮的真品药材基原鉴定提供分子依据。采用DNA克隆测序技术对从不同市场购得的丹皮样品及其伪品、混淆品的ITS2基因进行测序分析研究。丹皮与其伪品川赤芍、亳州芍药的rDNA-ITS2序列长度均为412bp。排序比较发现不同市场上的丹皮样品rDNA-ITS2序列变异位点丰富。鉴定出与丹皮真品(黄沙与白沙)及伪品的rDNA-ITS2序列完全相同的样品。其他样品的rDNA-ITS2序列与丹皮真品的差异明显。通过丹皮ITS2序列差异比较分析。ITS2序列可以作为鉴别丹皮药材真伪的一种手段。
rDNA-ITS2 sequence of Paeonia suffruticosa Andr and its counterfeit was analyzed. Genetic diversity of Paeonia suffruticosa Andr was studied rDNA-ITS2 sequence for the Paeonia suffruticosa Andr and its counterfeit was sequenced. The sequence length of Paeonia suffruticosa Andr and its counterfeit, is 412bp. There are plenty of variable sites in pairwise comparison of the sequence of Paeonia suffruticosa Andr. rDNA-ITS2 sequence is a method in identifying Paeonia suffruticosa Andr and its counterfeit.
出处
《生物学杂志》
CAS
CSCD
2008年第4期33-36,共4页
Journal of Biology
基金
安徽省自然科学基金(03043101)
安徽省优秀青年科技基金(04043056)
关键词
牡丹皮
ITS2序列
DNA测序
Paeonia suffruticosa Andr
ITS2 squence
DNA squencing
