摘要
目的探讨APOBEC3G在正常对照、慢性乙肝患者肝脏组织及肝癌组织中的表达情况,以及在慢性肝炎组织中与HBsAg、HBcAg表达的关系。方法收集正常对照肝组织9例、慢性肝炎肝脏组织37例,以及肝癌组织13例,运用免疫组织化学染色的方法检测APOBEC3G、HBsAg、HBcAg的表达。结果与正常肝脏相比,慢性乙肝肝炎患者肝脏组织中APOBEC3G的表达增强,差异具有显著性(P<0.05),主要定位于HBV感染的肝细胞及汇管区的炎性细胞胞浆中,但与同时在肝细胞内高度表达的HBsAg、HBcAg强度无明显相关性,r分别为0.04和-0.15,P>0.05。肝癌组织中APOBEC3G也有表达,但未明显强于癌旁组织(P>0.05)。结论HBV慢性感染肝细胞中表达增高的APOBEC3G未能有效控制病毒的活动,并可能与肝癌的发生无直接关系。
[Objective] To explore the expression of APOBEC3G in liver tissues from healthy controls, chronic hepatitis B and carcinoma and to investigate the relationship of APOBEC3G with HBsAg and HBcAg expression in HBV-infected liver tissues. [Methods] Collect liver tissues from 9 healthy controls, 37 chronic hepatitis B patients and 13 liver carcinomas. Determine the expression of APOBEC3G, HBsAg and HBcAg with immunohistochemistry. [Results] An increased expression of APOBEC3G was found in liver tissues from chronic hepatitis patients infected with HBV as compared to those obtained from HBV-negative control donors. The difference is significant （P 〈0.05）. APOBEC3G was primarily expressed in cytoplasm of HBV-infected hepatocytes and had no significant relationship with HBsAg and HBcAg which were also strongly expressed in these cells. （P 〉0.05） [Conclusion] APOBEC3G expressed in HBV-infected hepatocytes can not inhibit HBV replication effectively and might have no direct relationship with hepatocarcinoma formation.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2008年第15期2170-2172,共3页
China Journal of Modern Medicine
