摘要
目的了解中国农村地区65岁及以上老年人群 APOE 基因型频率分布,为研究 APOE 基因型频率分布与老年人群健康的关系以及开展防治老年性疾病提供科学依据。方法于2004—2008年4月,选择四川省邛崃市与剑阁县、山东省高密市与淄川区农村地区的老年人群(≥65岁)2000人为调查对象。采用 APOE 分型试剂盒对调查对象指血样品进行检测。结果我国农村地区老年人群 APOE 基因型ε2/ε2、ε2/ε3、ε2/ε4、ε3/ε3、ε3/ε4、ε4/ε4的频率依次为1.10%,13.55%,2.05%,68.80%,13.35%和1.15%;ε4携带者(ε2/ε4+ε3/ε4+ε4/ε4)与非ε4携带者(ε2/ε2+ε2/ε3+ε3/ε3)分别为16.55%和83.45%。山东省与四川省农村地区老年人群各 APOE 基因型频率间比较,经 x^2检验,差异均无统计学意义(P>0.05)。结论中国农村地区老年人群 APOEε4基因型频率分布较广。
Objective To know the apolipoprotein E （APOE） genotypes frequency of Chinese old people in the rural area. Methods 2 000 Chinese aged 65 years or older from four sites in China were enrolled in this study in 2004-2008. Two sites were from the Sichuan province in southwestern China, and another two sites were from the Shandong province in eastern China. The finger blood samples on filter paper were collected from all the investigated people in the end of the interview. The genotype for apolipoprotein E （gene symbol,APOE） was determined by eluting DNA from a dried blood spot,followed by HhaI digestion of amplified products. Results Through statistical analysis, APOE genotypes frequency of Chinese people aged 65 years or older in the rural area, as for ε2/ε2,ε2/ε3,ε2/ε4,ε3/ε3,ε3/ε4,ε4/ε4, they were 1.1%, 13.55% ,2.05% ,68.80%, 13.35% and 1.15% respectively. 4 carriers and no 4 carriers were 16.55% and 83.45%. Conclusion The distribution of APOE 4 genotype is more widely in Chinese people aged 65 years or older in the rural area.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2008年第8期662-664,共3页
Journal of Environment and Health
基金
美国NIH基金资助项目(R01 AG19181-01A2)
