摘要
目的：通过柱前衍生化后高效液相色谱法检测人血浆和尿液左卡尼汀含量。方法：采用Hypersil SiO2色谱柱，Waters2695高效液相色谱系统，用衍生化的方法处理血样和尿样，绘制标准曲线，测定精密度、回收率和稳定性，检测正常人血浆及尿液的左卡尼汀含量。结果：本方法血浆样品的回归方程为Y=0．02861X＋0．01190，r=0．9980，线性范围为5～320μmol·L^-1。尿液样品的线性回归方程为Y。0．0786X＋0．02620，r=0．9969，线性范围为5～320μmol·L^-1。RSD小于4％，回收率大于90％，稳定性良好。结论：本方法灵敏、准确、快速、简便，适用于临床同时检测血浆和尿液中左卡尼汀的浓度。
OBJECTIVE To establish an improved method in determination of plasma and urine L-carnitine by precolumn derivatization HPLC. METHODS The plasma and urine samples were derivatized, standard curves were drawn and the plasma and urine levels of L-carnitine were determined by HPLC with Hypersil SiO2 chromatographic column. RESULTS The regression equation of plasma was Y = 0. 028 61X ＋ 0.011 90, r = 0. 998 0, the regression equation of urine was Y = 0. （178 6X ＋ 0. 026 20, r= 0. 996 9. The precision RSD was less than 4%, the sample recovery was above 90%. The stability was good. CONCLUSION The improved method of determination plasma and urine L-carnitine by HPLC precolumn derivatization is sen sitive, fast, reliable and convenient and can be used for clinic determination.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2008年第15期1265-1268,共4页
Chinese Journal of Hospital Pharmacy
