摘要
以正常人血淋巴细胞染色体ＤＮＡ为模板，ＰＣＲ扩增出短体前β神经生长因子（ｐｒｅ－ｐｒｏ－β－ＮＧＦ）编码基因。将所得基因片段重组于Ｍ１３噬菌体载体，筛选得到含中国人ｐｒｅｐｒｏ－β－ＮＧＦ基因的克隆。采用Ｓａｎｇｅｒ单链末端终止法测出其全部的核苷酸序列，该序列与国外文献所报道的仅有一个碱基不同。将该基因亚克隆于真核表达载体ｐＥＶ１，经转染哺乳动物细胞ＢＨＫ后，在培养上清中检测到了成熟型β－ＮＧＦ。
With the chromosomal DNA of human blood lymphocytes as template,a chinese preproβnerve growth factor coding gene was amplified by PCR and recombinated into phage vector M13mp18.The whole nucleotides were sequenced by using Sanger's singlestranded DNA terminal termination method.The sequence of the cloned gene is completely the same as that reported abroad except only one base.The gene was subcloned into a eukaryotic expression vector pEV1 and mature βNGF was detected in the culture supernatant after the recombinant expression plasmid was transfected into mammalian cell BHK.
出处
《基础医学与临床》
CSCD
1997年第6期25-29,24,共6页
Basic and Clinical Medicine
