摘要
目的:研究大孔吸附树脂分离纯化鱼腥草总黄酮工艺,为鱼腥草总黄酮的工业化生产提供实验依据。方法:以贵州产鱼腥草为原料,以鱼腥草总黄酮含量及回收率等为考察指标,选用大孔吸附树脂对鱼腥草总黄酮进行分离纯化,分别采用静态试验、动态试验等考察大孔树脂对鱼腥草总黄酮的分离纯化效果及影响因素。结果:AB-8型大孔吸附树脂对鱼腥草总黄酮静态饱和吸附量为76.20mg.g-1(干树脂),洗脱率97.35%,动态饱和吸附量为74.5mg.g-1(干树脂),总黄酮回收率在82.8%、纯度在80%以上,是实验树脂中分离纯化鱼腥草总黄酮的最佳大孔吸附树脂。结论:分离纯化鱼腥草总黄酮最佳工艺条件为:AB-8型大孔吸附树脂,洗脱剂为70%乙醇,洗脱剂用量为2～3倍树脂体积,上柱总黄酮量与树脂比为1:13.4,上柱液总黄酮浓度为17.57mg.mL-1,流速2～3ml.min-1,上柱液pH4～5。
Objective： A method for separation and purification of flavonoids from Houstonian cordata Thunb with macroporous resin was studied, Method： By using Houstonian cordata Thunb in Guizhou and with the content and recovery rate of flavonoids as indexes and the static adsorption capacityand the static elution ratio. Result： The results are as flollows： The static adsorption capacity of AB-8 type resin was 76.20mg/g, the static elution ratio were 97.35%, the dynamic adsorption capacity of AB-8 type resin was 74.5mg/g, the recovery rate is more than 82.8% and the purity is more than 80%. AB-8 type resin is the best for separating and purificating Houstonian cordata Thunb in flavonoids. Conclusion： The optimum conditions is： AB-8 type macroperous resin, the eluant is 70% alcohol and 2-3 times as the volume of the resin, the volume of the resin is 13.4 times of flavonoids in sample, concentration of flavonoids of sample is 17.57mg/mland current velocity of 2-3ml/min, pH value of sample is 4-5.
出处
《中国民族民间医药》
2008年第8期7-11,共5页
Chinese Journal of Ethnomedicine and Ethnopharmacy
基金
贵州省"十一五"农业科技攻关项目
黔科合NY字[2006]3020
贵州省高层次人才科研条件特助经费项目
TZJF-2007年64号
关键词
大孔树脂
鱼腥草
总黄酮
分离
纯化
macropomus resin
Houstonian cordata Thunb
flavonoids
separation
purification
