摘要
用PCR方法从拟南芥和荠菜中分别克隆了DREB1A基因。序列分析发现从拟南芥中克隆的AtDREB1A基因与已发表的AtDREB1A基因序列(DQ372533)的同源性为99.69%,首次从荠菜中克隆的CbDREB1A基因序列(EF156749)与DQ372533的同源性为99.54%。利用这两个基因分别构建了两个诱导表达载体(prd29A/AtDREB1A,prd29A/CbDREB1A)和两个组成型表达载体(pCaMV35S/AtDREB1A,pCaMV35S/CbDREB1A),以便进一步开展植物抗旱基因工程研究。
The AtDREB1A and CbDREB1A genes were isolated from Arabidopsis thaliona and Capsella bursapastoris respectively by the method of PCR. Sequencing analysis of AtDREBIA indicated that the cloned fragment showed 99.69% identity to the published sequence （DQ372533）. The sequence of firstly cloned CbDREBIA gene （EF156749）shared 99.54% identity with that of DQ372533. By using of AtDREBIA and CbDREBIA genes,two induced expression vectors（prd29A/AtDREB1A,prd29A/CbDREB1A）and two constitutive expression vectors（pCaMV35S/AtDREB1 A,pCaMV35S/CbDREB1A）in plant were constructed, The obtained expression vectors can be applied on plants genetic engineering for drought-tolerance improvement,
出处
《生物技术通报》
CAS
CSCD
2008年第4期99-103,共5页
Biotechnology Bulletin
基金
中国科学院知识创新工程重要方向项目(KZCX3-SW-434)
湖南省杰出青年基金(03JJY1004)
关键词
DREB1A基因
拟南芥
荠菜
基因克隆
表达载体构建
DREBIA gene Arabidopsis thaliana Capsella bursa-pastoris Gene cloning Expression vector
