摘要
目的探讨加味大柴胡汤在临床上辅助治疗阻塞性黄疸的理论机制。方法制作阻塞性黄疸大鼠模型，分为7、14、21d共3个时段，每个时段分3组，即：（1）假手术＋生理盐水组（SO＋NS组）；（2）胆总管结扎＋生理盐水组（BDL＋NS组）；（3）胆总管结扎＋加味大柴胡汤组（BDL＋MMDB组）。每组6只SPF级SD大鼠，在各时段取大鼠肝组织及下腔静脉血，检测AST、ALB及TB；用RT．PCR检测TLR4mRNA的表达。结果胆总管结扎7d时，BDL＋MMDB组与BDL＋NS组外周血TB、AST比SO组均上升，且后者明显。BDL＋MMDB组与SO组及BDL＋NS组相比，差异均有统计学意义（P〈0．01）。胆总管结扎14、21d时，BDL＋MMDB组与BDL＋Ns组外周血TB、AST持续升高，且后者增高更明显。BDL＋MMDB组与SO组及BDL＋NS组相比，差异均有统计学意义（P〈0．01）。正常肝组织中TLR4mRNA的表达不明显。胆总管结扎7、14、21d时，BDL＋MMDB组与BDL＋Ns组TLR4mRNA表达持续显著增加，且后者增加更明显。同时段中BDL＋MMDB组与SO组相比、BDL＋MMDB组与BDL＋NS组相比，差异均有统计学意义（P〈0．01）。结论加味大柴胡汤可以通过降低肝组织中TLR4，从而减轻内毒素所致肝脏的损伤。
Objective To research the mechanism of impairment that cholestatic jaundice is treated assistly by MMDB. Methods Obstructive jaundice models with SD rats, which were divided into three time points randomly, namely 7,14,21 days, were established. In each time point, there were three groups （ each six rats） ： sham operation（ SO ＋ NS） ; BDL ＋ MMDB; （ BDL ＋ NS）. The rats were sacrificed at different time point, in order that liver tissue and inferior vena eava blood were taken out. The concentration of serum glutamate transaminase（AST）, albumin （ ALB ）, and total bilirabin （ TB ） were measured with biochemistry; the expression of TLR4mRNA was measured by reverse transcription PCR. Results On the 7th day after BDL, serum TB, AST level in BDL ＋ MMDB and BDL ＋ NS group were upgrade compared with SO group, in which BDL ＋ NS group were more obviously upgrade than BDL ＋ MMDB group （P 〈 0. 01 ）. There was different to compare between BDL ＋ MMDB group and SO group（P 〈0. 01 ）. On the 14th, 21st day after BDL, serum TB, AST contents were to last upgrade in BDL ＋ MMDB and BDL ＋ NS group. It was opposite to the SO group（P 〈0. 01 ）. Conclusion The level of TLR4 could be weaken by MMDB. It may relieve the impairment of liver in cholestatic jaundice by LPS.
出处
《国际外科学杂志》
2008年第8期518-521,共4页
International Journal of Surgery
基金
广东省科技攻关项目
广东省医学科研基金立项科课题（No.A2008475）
