摘要
【目的】了解广州地区儿童感染A群链球菌(GAS)的耐药情况及emm基因分型。【方法】对所分离的GAS以K-B法进行药敏试验并分析耐药情况;采用PCR法扩增GAS的M蛋白N末端,直接进行测序确定GAS的emm基因分型。【结果】GAS对青霉素、阿莫西林/棒酸、氧氟沙星、头孢菌素和万古霉素敏感,对红霉素、克林霉素、复方新诺明、四环素和氯霉素的耐药率分别为47.1%、42.5%、33.3%、21.8%、18.4%。以emm1基因型为主(37.9%),其次为emm18(13.8%),emm12(11.5%),emm69(9.2%)等。【结论】广州地区儿童感染GAS对大环内酯类等抗菌药物耐药形势严峻,应引起重视;GAS的emm基因型主要为emm1,选择主要流行菌株,可以作为基因疫苗的基础。
[Objective] To investigate the drug-resistance and emm gene genotypes of group A streptococcus（GAS） in children in Guangzhou. [Method] Antimicrobial susceptibility was tested using the Kirby-Bauer（K-B）trial and genotyping was performed by sequencing the PCR product of the emm gene fragment that encodes the N terminal of the M protein of GAS. [Results] The K-B trial showed all GAS strains were sensitive to mecillinam, cefditoren, ofloxacin and vancomycin while highly resistant to macrolide, tetracycline, chloramphenicol and sulfamethoxazole/trimethoprim. As to their genotype, the emml was the major genotype （37. 9%） in all the 87 strains. The followed was emm18（13. 8%）, emm12（11. 5%）, emm69 （9.2%）. [Conclusions] There is a high resistance to macrolies, therapy for GAS should choose penicillins at first. The study show the predominating emm gene genotype of GAS in Guangzhou is emml ,the result provides an useful guideline to develop the M-type specific vaccine.
出处
《中国儿童保健杂志》
CAS
2008年第4期389-390,共2页
Chinese Journal of Child Health Care
基金
广东省科技厅课题(56031)
关键词
A群链球菌
耐药率
emm基因
基因分型
group A streptococcus
drug-resistance rate
emm gene
genotyping
