摘要
目的：探讨不同剂量氨基葡萄糖（GS）对实验性自身免疫性脑脊髓炎（EAE）大鼠临床指标和基质金属蛋白酶-9（MMP-9）的影响。方法：用完全抗原和减毒百日咳杆菌原液免疫大鼠后随机分为三组，①EAE组（n=40）：腹腔注射4．5ml／kg·d^-1磷酸盐缓冲溶液；②GS1组（n=40）：腹腔注射90mg／kg·d^-1 GS溶液；③GS2组（n=40）：腹腔注射180mg／kg·d^-1 GS溶液。各组分别于免疫后（pi）6、8、10、12、14、16和18d取大鼠脑和脊髓制成石蜡切片，行HE染色和MMP-9免疫组化染色，进行组织学观察。结果：pi 14 d疾病发展达高峰，EAE组、GS1组和GS2组的发病率分别为77．8％、44．4％和11．1％（n=18），对三组发病率差异行χ^2检验有统计学意义（P〈0．01），两两相互比较有显著统计学差异（P〈0．05）。症状评分EAE组（2．33±1．57）与GS1组（1．00±1．24）和GS2组（0．60±0．89）行秩和检验比较差异，结果均有显著统计学意义（P〈0．05），GS1组与GS2组症状评分比较差异无显著性（P〉0．05）。同pi8d相比，EAE组、GS1组、GS2组体重分别平均减少（24．50±8．42）、（14．00±7．85）和（10．50±5．44）g。免疫组化染色MMP-9在脊膜细胞、炎细胞和内皮细胞内均呈阳性表达。pi 10 d EAE组MMP-9表达积分光密度达最大值，而GS治疗组pi 12 d MMP-9积分光密度表达达高峰。pi 6—16d GS治疗组MMP-9积分光密度与EAE组差异行方差分析，有显著统计学差异（P〈0．01）。pi 8—14 d GS2组与GS1组相比变化趋势也有统计学差异（P〈0．05）。结论：GS可抑制MMP-9的生成，对EAE发病具有一定程度的保护作用。
OBJECTIVE To investigate the immunosuppressive effect of glucosamine with different doses on matrix metalloproteinase- 9（MMP- 9 ）in rats with experimental autoimmune encephalomyelitis （EAE）. METHODS Complete antigen and pertussis toxin - immunized rats were divided randomly into three groups. EAE group （ n = 40 ） ： Phosphate - buffered saline 4. 5 ml/kg · d^- 1 was injected into abdominal cavity; Glucosamine 1 group（ n = 40 ）Glucosamine 90 mg/kg · d^- 1 was injected into abdominal cavity; Glucosamine 2 group （ n =40） ： Glucosamine 180 mg/kg · d^-1 was injected into abdominal cavity. The brains and spinal cords were removed for HE staining and the expression of MMP -9 wasdetected by immunohlstochemistry on the days of 6,8,10,12,14,16 and 18 pi. RESULTS The mean score of symptoms of glucosamine 1 group was 1.00 ± 1.24 and that of glucosamine 2 group was 0.60 ± 0.89, compared with 2.33 ± 1.57 of the EAE group. There were significant difference in incidence among three groups （EAE group, 77.8%, n = 18; glucosamine 1 group, 44.4%, n = 18; glucosamine 2 group, 11.1%, n = 18）. The expression of MMP -9 in the glucosamine group was lower than that in the EAE group. And expression of MMP -9 in glucosamine 2 group showed stronger inhibition when compared to that of glucosamine 1 group. CONCLUSION Glucosamlne may inhibit the production of MMP - 9, thereby protect BBB and ameliorate the infiltration of inflammatory cells in CNS.
出处
《天津药学》
2008年第4期1-5,共5页
Tianjin Pharmacy
基金
国家人口和计划生育委员会2005年科技立项(No.C1-42)
人事部06年度回国人员科技活动择优资助项目(No.2006-164)
