摘要
目的：探讨电离辐射对免疫细胞的影响及重组人铜锌超氧化物歧化酶（ｒｈＣｕＺｎＳＯＤ）的抗辐射作用。方法：观察６０Ｃｏγ射线５～７Ｇｙ照射前后不同时段给予ｒｈＣｕＺｎＳＯＤ对小鼠免疫细胞的影响。结果：电离辐射对小鼠免疫细胞有明显的损伤作用。与照射对照组比较，ｒｈＣｕＺｎＳＯＤ可减轻电离辐射对小鼠免疫细胞的影响，其作用包括增强脾Ｔ淋巴细胞对刀豆蛋白Ａ诱导的增殖反应，提高脾自然杀伤细胞的活性，升高全脾淋巴细胞及外周血白细胞和淋巴细胞的数量。辐射前后给药组的效果优于辐射前或辐射后给药组。结论：ｒｈＣｕＺｎＳＯＤ对防止免疫细胞的辐射损伤有一定作用。
Aim: To investigate the radioprotective effect of recombinant superoxide dismutase (rhCuZnSOD) in irradiation damaged mice model. Method: The mice were given rhCuZnSOD before and/or after whole body 5￣7 Gy γ ray irradiation and the changes of immunocyte activity and count number were observed and compared in differently treated groups of these mice. Results: As compared with those of the irradiation control groups, the rhCuZnSOD showed significant beneficial effects on immunocytes, these included enhancing the proliferative response of T lymphocyte to concanavalin A and the activity of natural killer cells in spleen, increasing the number of whole spleen lymphocyte, leucocyte and lymphocyte counts in peripheral blood. Conclusion: rhCuZnSOD has radioprotective effect on irradiation damaged mice especially when it is given both before and after irradiation.
关键词
辐射损伤
重组
铜锌
超氧化物歧化酶
免疫细胞
Radioprotection
Superoxide dismutase
human recombinant
Immunocyte
Free radicals
