摘要
采用生长曲线法测定了河流弧菌在牙鲆表皮、鳃和肠粘液中的生长情况,结果表明,河流弧菌在3种粘液中都能生长,生长前期符合Logistic模型,然后进入Gompertz模型,在鳃粘液中的生长量高于肠粘液和表皮粘液。采用毛细管法研究了河流弧菌对牙鲆3种粘液的趋化作用,结果表明,河流弧菌对牙鲆3种粘液具有较强的趋化应答,在粘液总蛋白含量相同的情况下,河流弧菌对牙鲆鳃粘液的趋化应答显著强于对表皮粘液的趋化应答(P<0.05),而对肠粘液的趋化应答极显著低于对鳃粘液和表皮粘液的趋化应答(P<0.01)。采用3H-TdR示踪法进行了河流弧菌对牙鲆3种粘液的粘附作用研究,结果表明,河流弧菌对牙鲆表皮粘液和鳃粘液的粘附率较高,而对肠粘液的粘附率较低,特别是孵育30、60和90min时河流弧菌对牙鲆表皮粘液和鳃粘液的粘附率显著高于对肠粘液的粘附率(P<0.05)。这些结果表明,河流弧菌能够在牙鲆粘液,尤其是鳃粘液中生长和定植,有利于其进一步感染、致病。
The growth of Vibrio fluvialis in skin, gill and intestinal mucus of Paralichthys olivaceus, the chemotactic response and adhesion of V. fluvialis to the mucus were investigated. The results show that, V. fluvialis grew well in all the mucus in Logistice growth model at prophase and Gompertz growth model at anaphase, the maximum biomass yield in gill mucus was higher than those in skin and intestinal mucus; V. fluvialis exhibited strong chemotactic response towards the mucus from the three organs, the chemotaxis to gill mucus was significantly stronger than to skin mucus (P〈0.05), and extremely significant lower (P〈0.01) to intestinal mucus than those to gill mucus and skin mucus. The bacteria adhesion to skin mucus and gill mucus were significantly higher (P〈0.05) than that to intestinal mucus after incubation for 30, 60 and 90 min: Therefore, pathogenic V. fluvialis could grow and colonize in mucus of P. olivaceus, especially in gill mucus, causing further infection.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2008年第4期362-367,共6页
Oceanologia Et Limnologia Sinica
基金
国家高技术研究发展计划(863计划)项目资助
2007AA09Z115号
福建省科技计划项目
2006F5066号
厦门市海洋与渔业局科技项目
200707号
关键词
河流弧菌
牙鲆
粘液
趋化作用
粘附作用
Vibrio fluvialis, Paralichthys olivaceus, Mucus, Chemotaxis, Adhesion