摘要
目的:探讨人与猪胆总管弹性模量的关系,为猪到人异种肝移植提供理论依据。方法:获得5例成年人尸体和50例3￣12月龄湖北白猪的胆总管,在软组织生物力学试验机上测定胆总管的压力-直径关系,计算出增量弹性模量(Einc)、压力-应变弹性模量(Ep)和容积弹性模量(Ev)。横断取材,冰冻切片,HE法染色,用计算机图像分析系统测量其管径和壁厚。结果:人与各月龄组猪胆总管的Einc(F=502.08,P=0.00)、Ep(F=137.42,P=0.00)和Ev(F=134.59,P=0.00)差异有统计学意义;7～10月龄猪胆总管的弹性模量与成人的相近(P>0.05),而3～6月龄及11、12月龄猪的弹性模量比成人的大(P<0.01)。结论:7～10月龄猪胆总管的生物力学特性与成人的比较接近,故在猪到人异种肝移植时,选用7～10月龄猪的肝脏作为供体材料,也许能提高移植肝的存活率。
Objective： To explore the relationship of elastic modulus of the common bile duct （CBD） between the human and pig and provide theoretical basis for pig-to-human liver xenotransplantation. Methods： The CBDs of 5 adult corpses and 50 Hubei white pigs aged between 3-12 months were obtained. The pressure-diameter relationship of the CBD was measured on the biomechanical testing system for soft tissues, while the incremental elastic modulus （Einc）, pressure-strain elastic modulus （Ep） and volume elastic modulus （Ev） were calculated. The materials were transversely extracted, refrigerated, and sliced up for HE staining. The diameter and wall thickness of CBD were measured by computer image analysis system. Results： There was significantly difference in Einc（F=502.80, P=-0.00）, Ep （F=137.42, P=0.00）, and Ev （F=134.59, P=-0.00） of the CBD between human and pigs of different months. The elastic modulus of CBD of pigs aged between 7-10 months was similar to that of the adult human （P 〉 0.05）, while the results of pigs aged between 3-6 months and 11-12 months was significantly higher than that of adult human （P〈0.01）. Conclusions： The biomechanical properties of the CBD of pigs aged between 7-10 months was similar to that of the adult human, thus, on pig-to-human liver xenotransplantation, the liver of pigs aged between 7-10 months should be chosen as the donator, which maybe increase the livability of transplanted liver.
出处
《中国临床解剖学杂志》
CSCD
北大核心
2008年第4期433-436,共4页
Chinese Journal of Clinical Anatomy
基金
湖北省教育厅科学技术研究项目(99A112)
郧阳医学院创新团队项目(2005CXX03)
关键词
人
猪
胆总管
弹性模量
生物力学
异种移植
human
pig
common bile duct
elastic modulus
biomechanics
xenotransplantation
