摘要
目的分析山西地区汉族人群T细胞免疫球蛋白域黏蛋白域蛋白-3(TIM-3)启动子区-574G/T位点单核苷酸多态性,探讨其与变应性鼻炎易感性之间的关系。方法分别采用等位基因特异性聚合酶链反应检测山西地区132例变应性鼻炎患者和120名健康者TIM-3启动子区-574G/T的单核苷酸多态性,计算基因型和等位基因频率。结果山西地区健康人群TIM-3启动子区-574G/T位GG、GT和TT基因型频率是0.990、0.010和0,而变应性鼻炎患者为0.940,0.060和0,其基因型和等位基因频率均与对照组差异有统计学意义(P=0.046,P=0.048)。结论山西汉族人群TIM-3启动子区存在多态性变异,其中-574G/T单核苷酸多态性可能与山西地区汉族成人变应性鼻炎易感性有关。
Objective To investigate single nucleotide polymorphism sites of the promoter region in T cells immunoglobulin domain and mucin domain protein-3 （TIM-3） and detect their relationship with allergic rhinitis in a population of adult Hans from Shanxi province of China. Methods The polymorphisms were detected by allelic specific polymerase chain reaction （AS-PCR）. The genotype and allele frequencies were calculated and analyzed. Results The genotype frequencies of GG, GT and TT in-574T/G polymorphism were 0.992,0.008 and 0 respectively in the healthy population and were 0.941,0.059 and 0 respectively in the allergic rhinitis population. There was significant difference in genotype and allele frequencies between the allergic patients rhinitis and control subjects （P = 0.046, P = 0.048）. Conclusion There are polymorphism sites of the promoter region in TIM-3, and one of these sites, the-574 G/T polymorphism site may be associated with allergic rhinitis in the population of adult Hans from Shanxi province of China.
