摘要
目的:探讨胰腺癌组织中Syk和VEGF-D蛋白的表达及临床意义。方法:应用免疫组化技术检测40例胰腺癌组织中Syk和VEGF-D蛋白的表达情况。结果:正常胰腺组织表达Syk蛋白,在慢性胰腺炎组织中有中等表达,其阳性率分别为100.0%(8/8)和77.8%(7/9);在胰腺癌组织中阳性率为27.5%(11/40),大多数呈中等或弱阳性表达,与前两者比较差异均有统计学意义,P值分别为0.0002和0.0079;Syk蛋白与肿瘤的发生部位、分化程度和有无淋巴结转移无关;与TNM分期有关,Ⅰ～Ⅱ期组表达明显高于Ⅲ～Ⅳ期组,P=0.0245。胰腺癌组织中VEGF-D蛋白阳性率为55%(22/40),肿瘤周边部位显著高于肿瘤中心部位,差异有统计学意义。有淋巴结转移患者的VEGF-D表达显著高于无淋巴结转移患者的表达,P=0.0253。Syk和VEGF-D的表达具有相关性。结论:Syk缺失后,VEGF-D表达增加,胰腺癌淋巴转移和浸润性增强。
OBJECTIVE.. To investigate the expressions of Syk and VEGF-D in pancreatic cancer and their clinical significance. METHODS.. The expressions of Syk and VEGF-D were assayed by means of immunohistochemistry in 40 pancreatic carcinomas. RESULTS： The positive rates of Syk were 27. 5%, 77. 8% and 100.0%, respectively in pancreatic cancer, chronic inflammtion and normal tissues. The expression of Syk in pancreatic cancer was significantely lower than that in other two kinds of tissues. There was no correlation between the expression of Syk and the site, differenti ation,metastasis. Ⅰ-Ⅱ stages of pancreatic cancer showed stronger expressions of Syk than Ⅲ-Ⅳ stages of pancreatic. The positive rate of VEGFD was 55%（22/40） in pancreatic cancer. The expression of VEGF-D in cancerous invasive edge was significantely higher than that in the center of cancerous tissues. There was no correlation between the expression of VEGF-D and the site, differentiation, histologial types. The expression of VEGFD in the patients with lymph node metastasis was higher than that in ones with out lymph node metastasis, P=0. 025 3. The low expression of Syk was consistent with the high expression of VEGF-D. CONCLUSION： The expression of VEGF-D rises after the loss of Syk, and the lymph metastasis and invasion of pancreatic cancer increase.
出处
《中华肿瘤防治杂志》
CAS
2008年第15期1166-1168,共3页
Chinese Journal of Cancer Prevention and Treatment
基金
国家自然科学基金(30603003)
山东省中青年科学家科技奖励基金(2005BS03003)
关键词
胰腺肿瘤
免疫组织化学
蛋白质酪氨酸激酶
血管内皮生长因子
淋巴结
pancreatic neoplasms
immunohistochemistry
protein-tyrosine kinase kinase
vascular endothelial growth factor
lymph nodes
