摘要
采用Alcalase及Neutrase蛋白酶酶解花生粕中的蛋白,制取花生抗氧化活性肽。在相同条件下,通过测定水解度得出Alcalase的水解效果比Neutrase好。通过正交试验得出Alcalase最佳水解条件为:底物质量分数8%,酶加量4%,pH 8.0,温度55℃,时间2 h。在最佳条件下水解度为17.95%。通过油脂的抗氧化试验,证明花生抗氧化活性肽具有明显的抗氧化活性,5%的花生抗氧化活性肽抗氧化效果与3%的VE抗氧化效果相当。
Alcalase and Neutrase enzyme were used to hydrolyze peanut meal to prepare antioxidative peanut peptide. Under the same conditions, the hydrolysis capability of Alcalase was h.igher than that of Neutrase through testing the hydrolysis degree. Orthogonal experiment showed that the optimal conditions of Alcalase were substrate concentration 8%, enzyme dosage 4%, pH 8.0, temperature 55 ℃, time 2 h. The hydrolysis degree was 17.95% under the optimal conditions. The antioxidative test of oil showed that peanut peptide had obvious antioxidative property, the antioxidant activity of 5% peanut peptide was equal to that of 3% VE.
出处
《中国油脂》
CAS
CSCD
北大核心
2008年第6期15-18,共4页
China Oils and Fats
关键词
花生粕
抗氧化
活性肽
peanut meal
antioxidation
active peptide
