摘要
目的研究雷米普利(RAM)对糖尿病大鼠心肌缺血/再灌注损伤的保护作用。方法链脲佐菌素致糖尿病大鼠被随机分为缺血/再灌注(I/R)、缺血预适应(IPC)和RAM3组。RAM组每天用RAM(1mg·kg-1)灌胃,IPC和I/R组用等体积生理盐水灌胃。4wk后各组动物均经历心肌缺血/再灌注损伤,IPC组于缺血前行心肌缺血预适应。连续监测心电图,检测心肌梗死范围、心肌细胞凋亡、凋亡蛋白Bcl-2与Bax表达,光镜下观察心肌形态学改变。结果与I/R组比较,RAM及IPC组ST-段抬高幅度降低,室早出现时间推迟,持续时间缩短,室速、室颤发生率降低,心肌梗死范围缩小,心肌细胞凋亡减轻,Bcl-2/Bax比值升高。结论连续4wk应用RAM可减轻糖尿病大鼠心肌缺血/再灌注损伤。
Aim To investigate the effect of ramipril on myocardial ischemia-reperfusion injury in diabetic rats. Methods Streptozotocin induced diabetic rats were divided randomly into ischemia/reperfusion （I/R）, ischemic preconditioning （ IPC ） and ramipril （ RAM ） groups. Rats in RAM group were administered RAM （ 1 mg·kg^-1） orally for 4 weeks, the others were administered normal saline. Then all rats were subjected to myocardial ischemia/reperfusion injury. Rats in IPC group were preconditioned before ischemia. ECG, myocardial infarct size, apoptosis and expressions of Bcl-2 and Bax, changes of myocardial morphology were examined. Results Compared with those of I/R group, the elevation of ST segment and the incidence of ventricular tachycardia and ventricular fibrillation during ischemia were significantly decreased, myocardial infarct size and cardiomyocyte apoptosis index were remarkably reduced,ratio of Bcl-2/Bax was increased in both IPC and RAM groups. Conclusion Myocardial ischemia/ reperfusion injury was decreased in diabetic rats after ramipril orally administered for 4 weeks.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2008年第7期924-928,共5页
Chinese Pharmacological Bulletin
基金
天津市科委自然科学基金资助项目(No05YFJMJCO3700)
天津医科大学重点基金资助项目(No2004xk29)
天津医科大学科研基金资助项目(No2004ky15)
关键词
雷米普利
糖尿病
缺血/再灌注
凋亡
缺血预适应
ramipril
diabetes mellitus
ischemia/reperfusion
apoptosis
ischemic preconditioning
