摘要
RACE:cDNA末端快速扩增技术进展明洪黄秉仁中国协和医科大学基础医学院中国医学科学院基础医学研究所国家分子生物学重点实验室北京100005基因表达水平和模式的变化驱动着生物体内主要的生物学过程。分离和克隆基因是研究基因结构、功能以及表达的基础。以...
Construction and screening of cDNA libraries for genes of interest represent the standard but tedious cloning procedures. The advent of PCR technology has greatly enhanced the efficiency of traditional gene isolation and cloning techniques.RACE (Rapid Amplification of cDNA Ends) is a simple, efficient, and sensitive technique for amplification of cDNAs encoding molecules with 5′ or 3′ regions of unknown sequence. Various modifications and improvements of the RACE procedure have been successfully employed.Here we reviewed the historical perspective, modified protocal and application of RACE.
出处
《生物工程进展》
CSCD
1997年第5期7-13,共7页
Progress in Biotechnology