摘要
在分析了新组合菌系ＳＣＢ３２９－ＳＣＢ９３３发酵过程特征的基础上，对流加发酵工艺中的种子培养、ｐＨ、溶氧的控制，以及发酵液初始培养基中的Ｌ－山梨糖浓度和流加起始点进行了优化，获得了比分批发酵更为满意的结果：发酵最终总糖达１３％（ｗ／ｖ）左右，发酵周期４０～５０ｈ，产２－酮基－Ｌ－古龙酸达１１５－１３０ｍｇ／ｍｌ，克分子转化率达８８ｍｏｌ％左右。
A novel component bacterial system, Gluconobacter oxydans SCB329 Bacillus thuringiensis SCB933 had the ability of high and stable yield.Based on the analysis about the charcteristics of the 2 keto L Gulonic fermentation process,the control method of the fermentation conditions,such as seed cultivaton,pH,dissolve oxygen(DO),L sorbose concentration and the beginning point of L sorbose feeding,were optimized.The results indicated that the total L sorbose concentraton reached about 14%(w/v);after a 40 50 hours'cultivation,the final concentration of 2 keto L Gulonic in the fermentation broth was about 115 130mg/ml;the conversion rate of 2 keto L Gulonic was about 88 mol%.
出处
《工业微生物》
CAS
CSCD
1997年第3期6-10,共5页
Industrial Microbiology
关键词
维生素C前体
酮基古龙酸
山梨糖
发酵
杆菌
Gluconobacter oxydans SCB329
Bacillus thuringiensis SCB933
2 keto L gulonic
Fed batch fermentation
Control
