摘要
目的通过浆膜腔积液细胞图片调查了解各级医院常规浆膜腔积液细胞检验现状,提高浆膜腔积液细胞检验质量。方法经浓缩、推片及瑞特-吉姆萨混合染色后摄制细胞图片,精选细胞图片40张,随机分发给浙江省内的各级医院,让其对40张细胞图片进行识别并反馈结果。此调查在第1次进行后进行讲解及培训,再间隔进行第2次、第3次调查。结果3次调查结果发现除核异质细胞、细菌、多倍体及淋巴瘤细胞回报正确率仍较低外,其余回报正确率均有明显提高(P<0.01),所有参加单位的平均回报正确率从首次的43.2%上升到第3次的67.3%(P<0.01)。特别是连续参加的49个单位正确率上升较快,其中三甲医院的准确率由45.4%上升至72.6%,三乙医院由44.9%上升至68.3%,二级医院由38.6%上升至65.9%。结论通过前后3次图片调查及质量控制说明基层医院常规浆膜腔积液细胞检测存在的问题应引起重视,细胞图片质控是提高基层医院常规浆膜腔积液细胞检测技能的一个值得推广的有效方法。
Objective To promote the quality of effusion cells examination in clinical laboratory by using quality control photographs. Methods 40 effusion cells smear photograghs of different cell types were prepared and distributed to the hospitals in ZheJiang Province at random. The hospitals were asked to recoganize the 40 cell pictures and return to us the results. After the first survey, we held an explanation and training. Then the second and the third surveys were made. Results The exactitude rates of the three serveys results increased remarkably except those of the polyploid and nucle us heterogenic cell （P 〈 0.01 ）. The exactitude rate of the average response increased from 43.2% for the first time to 67.3% for the third time （P 〈0.01 ）. In particular, the 48 hospitals which continuously took part in the survey, had enhanced the right ratio quickly,in which the numbers of class three A hospitals had risen from 45.4% to 72.6% , class three B from 44.9% to 68.3%, others from 38.6% to 65.9%. Conclusions The existing problem with identifying routine effusion cell in basic hospitals should draw our attention. Application of quality control material of typical cell pictures is an effective method to improve quality of the routine detection of effusion cells.
出处
《检验医学》
CAS
北大核心
2008年第4期425-427,共3页
Laboratory Medicine
基金
浙江省医药卫生科学研究课题资助项目(2006B06)
关键词
浆膜腔积液
细胞
图片
质量控制
Pleural fluid
Ascitic fluid
Cell picture
Quality control
