摘要
从256个自然试样中筛选到1株高效转化D-木糖为木糖醇的酵母菌株441-28-1。初始木糖质量浓度为90 g/L的条件下,24 h内的木糖利用效率为3.0 g/(L.h)。通过高效液相分析,菌株441-28-1的主要代谢产物为木糖醇。在初始木糖质量浓度为65 g/L的条件下,摇瓶分批发酵,木糖醇生成速率达1.1 g/(L.h),木糖醇转化率为70%。经过形态、生理生化特征测定,以及ITS序列分析(GenBank的登记号为EU121523),将441-28-1菌株鉴定为热带假丝酵母(Candida tropicalis)。Candida tropicalis(热带假丝酵母)已保存于中国高校工业微生物资源数据平台,保藏编号CICIM Y0092。
One yeast strain isolated from 256 natural samples was used for D-xylose. With the initial xylose concentration of 90 g/L, the xylose-utilizing efficiency was 3 g/（L-h） within 24 h. HPLC analysis showed that the main metabolic product was xylitol. With the initial xylose concentration of 65 g/L, the xylitol yield was 0. 7 g/g and the volume productivity was 1.1 g/（L-h）. According to the morphological characteristics, assimilation physiological tests and analysis of ITS nucleotide sequence （GenBank accession number EU121523 ） , the strain was identified as a strain of Candida tropicalis. The strain was submitted to the Culture and Information Centre of Industrial Microorganisms of China Universities and the corresponding serial number was CICIM Y0092.
出处
《生物加工过程》
CAS
CSCD
2008年第4期56-60,共5页
Chinese Journal of Bioprocess Engineering
基金
国家自然科学基金资助项目(20706024)
国家863计划资助项目(2007AA10Z359)
