摘要
目的研究动脉粥样硬化(atherosclerosis,AS)时动脉血管平滑肌细胞大电导的钙激活钾通道(Large-conductance Ca2+-activated K+channel,BKca)β1亚单位(KCMB1)基因mRNA定量表达,探讨KCMB1基因在AS发生中的作用。方法建立兔AS模型,应用逆转录-聚合酶链反应(RT-PCR),检测AS时胸主动脉KCMB1基因的mRNA表达变化。结果大电导的钙激活钾通道KCMB1基因在动脉粥样硬化组胸主动脉中表达明显减少。结论大电导的钙激活钾通道β1亚单位调节血管平滑肌细胞的收缩,可能与AS的发生有一定的关系。
Objective To study the KCMB1 gene mRNA expression level of Large-conductance Ca^2＋-activated K^＋ channels in vascular smooth muscle from atherosclerotic rabbit and its role in AS.Methods The AS was established in 10 New Zealand rabbits and another 10 rabbits were used as controls.Experimental group rabbits were red with high fat fodder.The rabbits in the control group merely feed on ordinary fodder.Both groups had been fed for 12 weeks.The real-time fluorescence quantitative PCR method was used to analyze the expression level of KCMB1 gene.Results The expression of KCMB1 gene mRNA is lowered in the AS group than in control group.Conclusion KCMB1 regulates the contraction of vascular smooth muscle which is related to AS.
出处
《四川医学》
CAS
2008年第7期829-831,共3页
Sichuan Medical Journal
关键词
动脉粥样硬化
血管平滑肌
钙激活钾通道
基因表达
逆转录-聚合酶链反应
兔
atherosclerosis
real time RT-PCR
vascular smooth muscle cells
calcium-activated potassium channels
gene expression
rabbit
