摘要
目的因脐血干细胞增殖能力自我更新能力较强,故脐血造血干细胞生物学特性研究已经成为热点。本文探讨观察了所提抗原及其对人脐血细胞(CTL)诱导的特异性抗肿瘤作用。方法用密度梯度离心法提取人肿瘤抗原,并测定诱导的人脐血细胞CTL活性。结果小肠纤维肉瘤Ag诱导4天后,不同效价实验组CTL活性均有升高,效价1∶100的抗原诱导的CTL活性最强。乳腺癌抗原诱导8天后,效价1∶100实验组CTL活性显著提高,结论所提两种抗原效果良好,可明显诱导脐血CTL活性,以效价1∶100实验组最佳。
Objective To observe the specific antitumor effect of the CTL（cytotoxic T lymphogyte） from cord blood cells which was induced by the extracted antigen. Methods To detect the activity of CTL induced by the tumor antigen which was extracted by the density gradient centrifugation through the 3H-TdR incorporation test.Results CTL induced by the antigen titer of 1：100 had the stronger killing activity. Conclusion The extracted antigen is effective and can enhance the activity of the induced CTL.
出处
《中国实验诊断学》
2008年第7期903-904,共2页
Chinese Journal of Laboratory Diagnosis
关键词
肿瘤抗原
人脐血
CTL
tumor antigen
human umbilical cord blood
cytotoxic T lymphocyte
