摘要
目的筛选化疗敏感性不同的下咽鳞癌差异基因表达谱,探讨基因表达谱预测下咽鳞癌化疗敏感性的可能性。方法选取诱导化疗敏感和不敏感的下咽鳞癌各4例,分别提取mRNA,体外反转录为cDNA和cRNA,不同生物素标记和片断化后制成cRNA探针,与人类基因组芯片杂交,对杂交信号扫描并归一化处理,使用GCOS4.0统计软件分析。以Signal Log Ratio≥1和change P-value≤0.05为标准筛选差异表达基因并根据生物学过程和功能分类。结果筛选差异表达基因119条,其中上调基因65条,下调基因54条。差异表达基因包括信号传导(STAT3),细胞周期(CCND1、TCF7L1),细胞凋亡(FADD),黏附分子(MUC4、cadherin 11、ICAM2)以及酶类等,化疗敏感组中EGFR、TGF-β和TNFR等细胞信号通路异常。结论不同化疗敏感性的下咽鳞癌基因表达谱不同,根据基因表达谱的变化评估化疗敏感性是可能的。
Objective Screening gene expression profiling from hypopharyngeal squamous cell carcinoma of different chemotherapy sensitivity and explore the possibility to predict the chemotherapy sensitivity by gene expression profiling. Methods Total RNA was isolated from four samples from chemotherapy sensitive and resistant hypopharyngeal squamous cell carcinoma respectively. Then synthesized into cDNA and cRNA in Vitro in turns. The cRNA probe, after biotin-labelled and fragmented, hybridized with human gene micro-array. The hybridization signal was scanned by the Gene Array scanner, analyzed by GCOS 4. 0 statistic software after normalization. The Signal Log Ratio≥1 and change P-value≤0. 05 were standard for identified significant difference genes. The gene was sorted out according to bio-process and function. Results Different gene expression profiling consisting of 119 genes was screened out. Among them,65 genes was up-regulated and 54 genes down-regulated, aberrant expressing genes were relative to signal transduction（STAT3 ）, cell cycle （ CCND1, TCF7 L1 ）, apoptosis （ FADD）, cell adhesion （ MUC4, cadherin 11 ,ICAM2）,enzyme（AKAPT,CDKN2A,MMP2） and so on. Signaling pathway of endepidermis growth factor receptor,TNF-β and STATs was dysfunction in the sensitive. Conclusion Hypopharyngeal squamous cell carcinoma of sensitive and resistant to chemotherapy had different gene expression profiling. It＇s possible to assess the sensitivity of hypopharyngeal squamous cell carcinoma before chemotherapy by gene expression profiling.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2008年第7期502-507,共6页
Cancer Research on Prevention and Treatment
关键词
下咽
鳞癌
化疗
敏感性
基因谱
Hypopharyngeal
Squamous cell carcinoma
Chemotherapy
Sensitivity
Gene expression profiling
