摘要
目的观察中药心复康对心肌梗塞后大鼠心肌血管新生的影响,探讨其对缺血心肌可能的保护机制。方法将大鼠随机分为假手术组、模型组、中药高剂量组和中剂量组,每组12只。模型组及中药组采用冠状动脉结扎法制作心肌梗塞模型。中药高剂量组(81 g.kg-1.d-1)和中剂量组(40.5 g.kg-1.d-1)给予中药心复康水煎剂灌胃,假手术组和模型组每天等量生理盐水灌胃。给药4周后处死大鼠,通过免疫组化方法,测定血管内皮细胞粘附分子CD31的表达以了解缺血心肌血管新生情况,检测血管内皮生长因子(VEGF)、碱性成纤维细胞生长因子(bFGF)的蛋白表达情况。结果中药高剂量组CD31的表达明显高于模型组,中药组VEGF及bFGF的表达量均明显高于模型组。结论心复康通过上调VEGF和bFGF的表达,能够有效促进血管新生,改善缺血心脏功能,挽救缺血的心肌。
Objective To study the effect of Xinfukang on angiogenesis after myocardial infarction （MI） in rats. Methods Myocardial infarction was induced by ligating the descending left coronary artery in male rats. Twenty four hours later ,48 survied rats with AMI were randomly divided into myocardial infarction control group, sham -operated group, Xinkukang middle dose group and Xinfukang high dose group. After 4 weeks of therapy,cardiac tissue section was observed and the expression of CD31 ,VEGF, bFGF was detected by immunohistochemistry. Results Compared with sham operated, Xinfukang high dose group could enhance expression of CD31, VEGF, bFGF. Conclusion Xinfukang can enhance angiogenesis after MI in rats and improve ischemia cardiac function.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2008年第5期1076-1077,共2页
Lishizhen Medicine and Materia Medica Research
基金
天津市卫生局课题(No.2005070)
关键词
心复康
心肌梗塞
血管新生
Xinfukang
Myocardial infarction
Angiogenesis
