摘要
液泡的活体标记是研究保卫细胞液泡动态的前提。结合作者的研究,介绍了利用丫啶橙(acridine orange,AO)、Lysotracker Red DND-99、二乙酸荧光素(fluorescein diacetate,FDA)、2',7'-二(羧乙基)-5(6)-羧基荧光黄(BCECF-AM)以及绿色荧光蛋白(green fluorescent protein,GFP)转基因等活体标记保卫细胞液泡的方法。研究结果表明,AO可以方便和快捷的标记保卫细胞液泡;FDA标记可以显示液泡的边界,也可以用于保卫细胞液泡的标记。利用转染GFP融合基因的方法也是标记保卫细胞液泡的最好选择。
Vital labeling of vacuole is required for the study of vacuolar dynamics. Several methods for vacuole labeling of guard cells in vivo were introduced in this paper by using of acridine orange, Lysotracker Red DND-99, BCECF-AM, fluorescein diacetate and GFP fusion protein. We found that the procedures of vacuolar labeling by acridine orange and fluorescein diacetate are simple and the results are better. In addition, GFP fusion protein might be the best strategy for vital vacuolar labeling in the future.
出处
《细胞生物学杂志》
CSCD
2008年第3期411-414,共4页
Chinese Journal of Cell Biology
基金
国家自然科学基金资助项目(No.30770193)~~
关键词
保卫细胞
液泡
活体标记
guard cell
vacuole
vital labeling
