摘要
本研究实现了墨汁鬼伞[Coprinus atramentarius(Bull.Fr.)Fr.]的液体培养,通过单因素试验研究了不同碳源、氮源及无机盐对墨汁鬼伞菌丝体生长和胞外多糖产量的影响。在此基础上,通过响应曲面法(RSM)研究了各营养因子的最佳水平,得到当葡萄糖、玉米粉、麸皮、KH2PO4和MgSO4·7H2O分别为13.51、17.07、7.62、2.29和2g/L时,胞外多糖最大预测值为293.73mg/L;当上述变量为10.07、18.02、11.63、1.58和4g/L时,菌丝体最大生长量为6.24g/L。
Coprinus atramentarius was cultivated with submerged fermentation method. Various nutritional factors including carbon sources, nitrogen sources and inorganic salt affecting the yield of extracellular polysaccharide （EPS） and mycelium growth were studied by single factor test. The key influential factors were further investigated by response surface methodology. By solving the quadratic regression model equation with appropriate statistic methods, the optimum concentrations for the submerged fermentation medium are determined that glucose, corn powder, wheat bran, KH2PO4 and MgSO·47H2O are 13.51, 17.07, 7.62, 2.29 and 2 g/L, respectively, and the predicted yield of the extracellular polysaccharide is 293.73 mg/L. When the above values are 10.07, 18.02, 11.63, 1.58 and 4 g/L respectively, the maximum biomass is 6.24 g/L.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第7期291-296,共6页
Food Science
关键词
墨汁鬼伞
液体发酵
胞外多糖
生物量
响应曲面法
Coprinus atramentarius
liquid fermentation
extracellular polysaccharide
biomass
response surface methodology
