摘要
用不同的试剂提取大黄中的有用成分,用比色法测定出大黄提取物对Fenton反应产生的羟自由基。Fenton反应产生的羟自由基与二甲亚砜反应,生成甲基亚磺酸,再与坚牢蓝BB盐反应形成偶氮砜,用比色法测定溶液的吸光度,发现大黄的提取物对羟自由基存在明显的清除作用,且与清除率有量效关系。
Different reagents were used to lixiviate rheum. Hydroxyl radicals produced in Fenton reaction can react with dimethyl sulfoxide to produce methane sulfinic acid,and further reacts with fast blue BB salt to form a light yellow diazosulfone. Hyderoxyl radicals can be indirectly determined by colorimetry. The hydroxyl radical can be removed by extracted rheum.
出处
《光谱实验室》
CAS
CSCD
2008年第4期614-617,共4页
Chinese Journal of Spectroscopy Laboratory
