摘要
利用1对兼并引物,从核桃(Juglans regia)中克隆得到苯丙氨酸解氨酶(PAL)基因cDNA片段,并命名为JrPAL,GenBank登录号为AY747676。JrPAL长866bp,编码289个氨基酸。通过核苷酸和蛋白质序列多重比较,发现JrPAL与其他植物的PAL基因高度同源。JrPAL编码的蛋白质序列包含与水稻、玉米PAL蛋白质相同的脱氨基位点和催化活性位点。PAL系统进化树表明,JrPAL与乔木类植物的PAL基因聚类关系最近。克隆JrPAL,可为利用基因工程技术调控核桃黄酮化合物的代谢提供基因资源。
A phenylalanine ammonia-lyase （PAL） gene cDNA was cloned from Juglans regia using a pair of degenerated primers. The PAL cDNA fragment of J. regia （designated as JrPAL） was 866 bp length and it encoded a 289 amino acid protein. JrPAL was found to have extensive homology with those of other plant PAL genes via multiple alignments. The deamination sites and catalytic active sites in PAL protein of Oryza sativa and Zea mays were also found in JrPAL. Phylogenetic tree analysis revealed that JrPAL had closer relationship with PALs from arbor plants than from other plants. JrPAL was a useful tool to study the regulation of flavonoid metabolism in J. regia.
出处
《湖北农业科学》
北大核心
2008年第6期622-626,共5页
Hubei Agricultural Sciences
基金
教育部新世纪优秀人才计划项目(NCET-04-0746)
湖北省自然科学基金项目(2002AB094)
湖北省自然科学青年杰出人才基金项目(2003AB014)
湖北省教育厅重大科技项目(Z200627002)
