摘要
目的探讨直肠癌中微淋巴管密度(MLVD)、微血管密度(MVD)与淋巴结转移及其生物学行为之间的关系。方法应用免疫组化法检测76例直肠癌和瘤周、远端切缘黏膜中D2-40和CD34阳性标记的微淋巴管和微血管数,并应用Western blot法检测7例直肠癌组织中D2-40蛋白含量。结果直肠癌总MLVD(15·66±4·82)、前缘区MLVD(18·26±2·36)显著高于瘤周、远端切缘黏膜MLVD(5·70±2·95、6·00±3·12,P<0·05),与Dukes′s分期、淋巴结转移、淋巴管受累呈正相关关系(P<0·05)。直肠癌总MVD、前缘区MVD与Duke′s分期相关(P<0·01)。结论微淋巴管密度,尤其在前缘区,与直肠癌的淋巴结转移及侵袭性生长有密切相关。干预直肠癌生长转移需要抑制血管生成的同时也要抑制淋巴管生成。
Objective To study the relationships between microlympha vessel density （MLVD） marked by D2-40 and microvascu- lar density （MVD） marked by CD34 and clinical biological features of rectal carcinomas. Methods MLVD and MVD were examined in 76 cases with rectal carcinomas, 42 matched adjacent normal mucosa and 76 distant normal mucosa samples by immunohistochemistry; D2-40 protein content in 7 primary tumors was detected by Western blot. Results MLVD in carcinoma tissue （ 15.66 ± 4.82 ） , in anterior field （18. 26-± 2.36） was significantly higher than that in adjacent mucosa （5.70 ± 2.95） and distant normal mucosa （6. 00 ± 3.12） （P 〈 0. 05） , were positively related to Dukes stages, lymph node metastasis, lymphatic vessel invasion （P 〈0. 05）. MVD in the whole areas and marginal areas were correlated with Dukes＇stage of rectal carcinomas. Conclusions MLVD, especially in the marginal areas, is associated with the lymph node metastasis and the procession of rectal adenocarcinomas. The growth and metastasis of rectal cancer could be intervened by inhibiting angiogenesis and lymphatic formation.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2008年第13期1267-1269,共3页
Chinese Journal of Gerontology
基金
河北省科技厅指令性计划项目(No·07276101D-50)
关键词
直肠肿瘤
微淋巴管密度
微血管密度
淋巴结转移
Rectal neoplasms
Micro-lymphatic vessel density
Microvascular density
Lymph node metastasis
