摘要
目的 探讨fractalkine受体CX3CR1基因多态V249I和T280M与颈动脉狭窄的相关性。方法 经颈部彩色多普勒超声检查诊断颈动脉狭窄患者318例，通过聚合酶链反应-限制性片段长度多态性（PCR—RFLP）和基因测序确定CX3CR1基因V249I和T280M多态性，并与292例无颈动脉狭窄者比较。结果 CX3CR1基因T280M多态中基因型MM和TM在颈动脉狭窄组明显低于对照组（分别为22．6％和31．2％，OR=0．646，95％CI 0．451—0．928，P=0．017），颈动脉狭窄组M等位基因频率明显低于对照组（分别为13．8％和19．2％，OR=0，677，95％ CI 0．499—0．918，P=0．010）。CX3CR1基因V249I多态中基因型Ⅱ和Ⅵ在颈动脉狭窄组和对照组差异无统计学意义（分别为39．0％和43．8％，OR=1．012，95％CI 0．731—1．403，P=0．940），颈动脉狭窄组Ⅰ等位基因频率明显低于对照组（分别为28．6％和32．7％，OR=0．842，95％ CI 0．660—1．076，P=0．034）。颈动脉狭窄组Ⅵ和Ⅱ在稳定斑块组明显增高（OR=0．610，95％ CI 0．387—0．962，P=0．033）。多因素Logistic回归分析显示MM和TM基因型是颈动脉狭窄的独立危险因素（OR=1．847，95％ CI 1．091—3．127，P=0．022）。结论 CX3CR1基因T280M多态中MM和TM基因型是颈动脉狭窄的一个独立危险因素，而V2491多态中Ⅱ和Ⅵ基因型与颈动脉粥样硬化斑块稳定性有关。
Objective To study the association of V249I and T280M polymorphisms of fractalkine receptor CX3CR1 with carotid artery stenosis （CAS）. Methods 318 patients with CAS diagnosed using color Doppler ultrasound criteria were studied and compared with 292 subjects without CAS. V249I and T280M polymorphic genotypes of CX3CR1 were determined by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） and sequencing analysis. Results The genotypes of MM and TM were associated with reduced risk of CAS, the frequency of genotype MM ＋ TM in the two groups being 22. 6% and 31.2% respectively （ OR = 0. 646, 95% CI 0. 451-0. 928, P = 0. 017） , the frequency of M allele was significantly lower in patients with CAS than in those without CAS （ 13.8% and 19. 2% respectively, OR = 0. 677, 95% CI 0. 499-0. 918,P =0. 010）. No differences were observed in the Ⅱ, Ⅵ, or VV genotype, the frequency of genotype Ⅱ ＋ Ⅵ in the two groups being 39. 0% and 43.8% respectively （ OR = 1. 012, 95% CI 0. 731-1. 403 ,P = 0. 940）, the frequency of I allele was significantly lower in patients with CAS than in those without CAS （28. 6% and 32. 7% respectively, OR = 0. 842, 95% CI 0. 660-1. 076, P = 0. 034 ）. The genotypes of II and VI in patients with stable plaques were more frequent than in vulnerable plaques（ OR =0. 610, 95% CI 0. 387-0. 962, P = 0. 033 ）. Multiple Logistic regression analysis revealed that the genotypes of MM and TM were an independent risk factor for CAS （ OR = 1. 847,95% CI 1. 091- 3. 127 ,P = 0. 022 ）. Conclusion The genotypes of MM and TM are independent risk factors for carotid artery stenosis, and the genotypes of Ⅱ and Ⅵ are associated with the stability of carotid artery plaques.
出处
《中华神经科杂志》
CAS
CSCD
北大核心
2008年第7期443-447,共5页
Chinese Journal of Neurology
关键词
颈动脉狭窄
颈动脉疾病
受体
趋化因子
多态现象
遗传
Carotid stenosis
Carotid artery diseases
Receptors
Chemokine
Polymorphisms, genetic
