摘要
以8个大花蕙兰品种茎尖为材料，采用不同培养基对茎尖原球茎进行诱导、增殖和分化培养，探讨不同浓度6-BA和椰乳对原球茎诱导、增殖和分化的影响效果。结果表明。低激素水平培养基1／2MS＋0．5mg／L 6-BA＋10％椰乳＋2％白糖＋适量活性炭对诱导产生原球茎的效果很好，大部分品种的原球茎诱导率达80％以上；在增殖培养基中添加0．5～1．0mg／L 6-BA和15％椰乳，可明显提高5个品种的原球茎诱导率和增殖率。将多次增殖的原球茎转入1／2MS＋1．0mg／L 6-BA＋0．5mg／L NAA＋20％椰乳＋1％白糖的分化培养基，原球茎可继续增殖并分化形成许多类胚性的单极丛生芽，再经分化培养后可获得大量根苗完整的再生植株。因此，试验结果可为大花蕙兰的高效低成本组培快繁途径提供依据。
The stem apexes of eight Cymbidiam grandiforium varieties were used as experiment materials, and the inducement, propagation and differentiation of protocorm of stern apex were carried out in different culture media to study the effects of different ooncentrations of 6-BA and coconut milk on them. The results showed that low hormone level culture medium with 1/2MS＋ 0.5mg/L 6-BA＋ 10%CM＋ 2%white sugar ＋ right amount activated carbon had better effect on the inducing of protocorm, the inducing rate of protomrm for most varieties were over 80%. Adding 0.5-1.0 mg/L 6- BA and 15 % coconut milk in multiplication culture medium could increase the inducing rate and multiplication rate of protocorm for five varieties. The protocorm which multiplied many times was transferred to differentiation culture with 1/2MS ＋ 1.0rng/L 6-BA ＋ 0.5mg/L NAA ＋ 20 % CM ＋ 1% white sugar, and the protomrm ~dd continue to multiply and differentiate, then form many multiple shoot clumps of embryonic body. After differentiation culturing, a great amount of plant regeneration with root and sprout were obtained. Therefore, the results could provide reference to the tissue culture and rapid propagation method of Cymbidiam grandiforium with high efficiency and low cost.
出处
《广西农业科学》
CSCD
2008年第3期284-286,共3页
Guangxi Agricultural Sciences
关键词
大花蕙兰
原球茎
增殖
分化
Cymbidiam grandiforium
protocorm
multiplication
differentiation
