摘要
以7种不同基因型西瓜为试材进行离体花药培养,对诱导愈伤组织的条件进行了研究。结果表明,西瓜离体花药在MS+KT1.5mg/L+6-BA2.0mg/L+NAA1.0mg/L,MS+KT1.5mg/L+6-BA2.0mg/L+NAA1.5mg/L,MS+KT0.5mg/L+6-BA0.5mg/L+NAA2.0mg/L等3种培养基上愈伤组织诱导率最高,其中野生种331号在MS+KT0.5mg/L+6-BA0.5mg/L+NAA2.0mg/L培养基上诱导率达到了68.42%。将长势较好的愈伤组织转到MS+三十烷醇2.0mg/L+6-BA0.5mg/L培养基上,76%的愈伤组织再生出丛生芽。丛生芽在MS+IBA0.5mg/L的培养基上长出了根,再将其转入1/2MS+IBA0.2mg/L+IAA1.0mg/L培养基上,获得了小植株。
The technology of anther culture was studied by using 7 watermelon varieties. More callus were formed on MS+KT 1.5 mg/L+6-BA 2.0 mg/L+NAA 1.0 mg/L, MS+KT 1.5 mg/L+6-BA 2.0 mg/L+NAA 1.5 mg/L and MS+KT 0.5 mg/L+6-BA 0.5 mg/L+NAA 2.0 mg/L. The wild watermelon 331 produced 68 callus per 100 anthers on MS+0.5 mg/L KT+0.5 mg/L 6-BA +2.0 mg/L NAA. When the selected callus were grown on MS+2.0 mg/L Triacontanol+0.5 mg/L IBA ,green shoot formed from the cullus. Plantlets were obtained by transferring the shoots to 1/2 MS+0.2 mg/L IBA+1.0 mg/L IAA.
出处
《中国瓜菜》
CAS
2008年第4期8-10,共3页
China Cucurbits And Vegetables