摘要
采用大鼠、小鼠脑缺血性缺氧以及结扎小鼠双侧颈总动脉造成的脑缺血再灌注损伤两种模型观察童智灵口服液对动物中枢神经系统的影响。并测定大鼠全血ＬＰＯ、ＳＯＤ和ＧＳＨＰｘ含量，以探讨其作用机制。研究结果表明，两种剂量灌胃给药，均可显著延长大、小鼠脑缺血性缺氧后的存活时间，并减少小鼠脑缺血４５ｍｉｎ再灌注６ｈ的卒中指数和２４ｈ内缺血再灌注损伤引起的死亡率。ｉｇ给药１５天，可降低大鼠全血ＬＰＯ含量，提高ＳＯＤ、ＧＳＨＰｘ活性，产生明显的抗氧化作用。说明，童智灵口服液可提高大、小鼠对脑缺氧的耐受能力并可对抗小鼠脑缺血及缺血再灌注损伤，对脑缺血小鼠具有保护作用。其作用机制可能与提高动物体内氧化酶活性，抑制自由基生成有关。
Using animal models of both cerebral anoxia and cerebral ischemiareperfusion injury,the effects of Tongzhiling oral liquor(TZLOL)on the cerebral anoxia and ischemia were studiedThe LPO level,and SOD and GSHPx activity in the rats blood were measeuredTZLOL(igadministration of two dosages once a day for 5 days in rats and mice)could significantly prolong the surviving time of animals after they have been suffered from cerebral ischemic anoxia,and also decrease the stroke index during 6 h after 45 min cerebral ischemiareperfusion injury as well as decrease the mortality in miceMeanwhile,TZLOL(igadministration for 15 days in rats)could decrease the LPO level and elevate the SOD and GSHPx activity in blood of ratsThe results show that TZLOL can increase the tolerance of animal cerebra to ischemia and anoxia,and be of an effect on the cerebral ischemiareperfusion injury and the effect of anntioxidantsThe protection mechanism on the anticerebral ischemiareperfusion injury may relate to its antioxidation
关键词
童智灵口服液
脑缺氧
脑缺血
抗氧化
药理
Tongzhiling oral liquor
cerebral anoxia
cerebral ischemiareperfusion injury
effect of anti oxidation
rats
mice
