摘要
本文同时采用分离和电镜扫描技术研究了光滑足距小蠹Xylosandrus germanus（Blandford）不同发育阶段坑道和贮菌器内真菌种类的组成和变化。Ambrosiella hartigii是光滑足距小蠹扩散、蛀孔、越冬和体壁新硬化的成虫贮菌器内唯一分离到的真菌,而刚羽化的雌成虫贮菌器中没有分离到任何真菌种类,体壁新硬化的成虫贮菌器中真菌的分离频率最低。坑道内共分离到4种真菌：A.hartigii,Acremoniumkiliense和2种镰刀菌Fusarium spp.。从卵期到蛹期的坑道中,A.hartigii总是能被分离到,占分离物总数的百分率较高,达40 %~60 %;而在成虫期坑道中,A.kiliense和Fusarium sp.1被分离率较高,都为34.6 %。扫描电镜观察表明,A.hartigii为卵期到蛹期坑道中的优势种,而镰刀菌Fusarium spp.则是成虫期坑道中的优势种。这些结果均表明,Ambrosiella hartigii,Acremonium kiliense和2种镰刀菌Fusariumspp.是光滑足距小蠹的虫道真菌,而且这些真菌种类组成和优势种类是随着蠹虫的发育阶段而变化的。真菌种类的组成和变化可能与幼虫和成虫的营养需求以及虫道真菌的生长特性有关。
The species composition and variety of the fungi in the gallery system and in the mycangium of Xylosandrus germanus （Blandford） at various developmental stages were investigated by isolation experiments and scanning electron microscopy. Ambrosiella hartigii Batra was the only fungus isolated from the mycangia of the dispersal, boring, overwintering and newly sclerotized adults but not the callow adults. The lowest frequency of the fungus isolated from the mycangium occurred in the newly sclerotized adults. The fungi isolated from galleries consisted of A. hartigii, Acremonium kiliense Gruetz and two species of Fusarium. A. hartigii occurred in a higher percentage of about 40% - 60% during the period from egg to pupal stage, while A. kiliense and Fusarium sp. 1 accounted for 34.6% and 34.6% from the adult galleries, respectively, A. hartigii was always isolated from galleries from the egg to pupal stage. Scanning electron microscopy showed that the conidiophores and conidia of A. hartigii were predominantly occurred at the bottom of galleries from the egg to pupal stage, whereas Fusarium spp. were predominantly occurred in the mature adult galleries. These results indicated that A. kiliense, two species of Fusarium as well as A. hartigii were associated with X. germanus and that the composition and the relative dominance of the fungi in the gallery system and in the mycangium varied with developmental stages of the beetle. This study suggests that the composition and variety of the fungi are likely related to nutritional requirement of larval and adult beetles as well as growth characteristics of the fungi.
出处
《昆虫学报》
CAS
CSCD
北大核心
2008年第6期595-600,共6页
Acta Entomologica Sinica
基金
四川省科技厅应用基础项目(2006J13-040)
关键词
光滑足距小蠹
虫道真菌
坑道
贮菌器
种类组成
优势种
Xylosandrus germanus
ambrosia fungi
mycangia
gallery
species composition
dominant species
