摘要
应用PCR方法扩增山羊痘病毒QL、LD、Y和B株ITR基因片段,插入pMD18-T载体,转化DH5α感受态细胞,提取重组载体经PCR和酶切鉴定后测序,并与GenBank上8株羊痘病毒相应序列进行同源性分析。结果经PCR扩增,4株病毒均可得到一条均一的DNA片段,该片段可被内切酶AluⅠ特异酶切,而且该PCR的DNA模板最小检出量为24.40pg。经测序,该基因片段长度为289bp;序列比较发现,4株病毒ITR片段与GenBank上2株山羊痘病毒的核苷酸与氨基酸序列同源性均为100%,与3株绵羊痘病毒分别为98.2%和96.5%,与3株牛疙瘩皮肤病病毒为98.2%～99.3%和96.5%～97.7%。这表明ITR基因片段在羊痘病毒属中较为保守,可以针对ITR基因建立羊痘病毒的PCR检测方法。
The inverted terminal repeat （ITR） gene fragments of goat poxvimses QL, LD, Y and B strains were amplified by PCR and sequenced. The ITR gene fragments of the four goat poxvimses strains were 289 bp in length and shared 100 % sequence identity with 2 strains of goat poxvimses in the GenBank, 98.2 % and 96.5 % with 3 strains of sheep poxvimses, and 98.2 %-99.3 % and 96.5 %-97.7 % with 3 strains of lumpy skin disease viruses, respectively. These results indicated that the ITR gene is highly conserved in the capripoxvims.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2008年第7期519-522,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
贵州省科技攻关项目(黔科合农社字2003NGY007号)
国家教育部科技重点项目(206132)
