摘要
用改良的技术方法研究高本底辐射诱发染色体畸变剂量效应关系。方法２８名受检者选自高本底和对照地区１０个家庭中的祖、父、子三代。个体累积剂量分别为２５．２～２４４．８和５．４～５１．７ｍＧｙ。分离淋巴细胞培养法制备染色体标本。每例分析２０００～３０００细胞。结果高本底地区家庭成员非稳定性染色体畸变率随年龄增长而增高（Ｙ＝０．６３２３＋０．０４６３Ｘ，ｒ＝０．８７５０）；并且其中年组和老年组平均畸变率显著高于对照组（２．５６ｖｓ．１．２５，Ｐ＜０．０５和３．６０ｖｓ．１．２２，Ｐ＜０．００１）。在对照地区未见到染色体畸变率与年龄增长相关。高本底地区个体畸变率和累积剂量间呈明显的直线相关（Ｙ＝０．７４４１＋０．０１５４Ｄ，ｒ＝０．８６）。结论高本底持续照射诱发的人体内非稳定性染色体畸变可随受照射时间的延长而持续升高，但当累积剂量降至约５０ｍＧｙ时，用目前方法进行定量分析是有困难的。
Objective\ To obtain a quantitative data regarding high background radiation induced human chromosome aberrations by using advanced techniques.Methods\ Environmental exposure dose for each individual was carefully measured.The estimated life time doses ranged 25.2-244.8 and 5.4-51.7mGy for individuals from the high background radiation area (HBRA) and the control area,respectively.Peripheral blood specimens were taken from 28 family members of three different generations in both areas.Purified lymphocyte culture technique for chromosome preparation was adopted and a total number of 70000 metaphases were scored.Results \ In the case of HBRA,the frequencies of Dic+Rc increased with age,but no age dependency was observed in subjects from the control area.The mean aberration yields significantly in excess of control value were seen in two older age groups.The aberration frequencies increased in proportion to the cumulative dose of the individuals living in HBRA.The estimated rate of increase per mGy was 1.5×10 -5 per cell.Conclusion\ It seems that Dic can continuously accumulated over life time chronic low dose exposure and can serve as a reliable biological indicator.When the dose reduces to about 50mGy, however,it becomes difficult to use the current method for quantitative analysis.
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
1997年第6期390-394,共5页
Chinese Journal of Radiological Medicine and Protection
关键词
高本底辐射
辐射
家庭成员
梁色体畸变
淋巴细胞
High background ionizing radiation\ Family member\ Chromosome aberrations\ Quantitative analysis
