摘要
目的观察高脂饮食诱导的非酒精性脂肪性肝炎(NASH)大鼠肝组织抵抗素mRNA的表达与定位,探讨其在NASH发病中的作用。方法16只雄性Wistar大鼠被随机分为普通饲料和高脂饲料喂养组,连续18周后处死各组大鼠,测定血清甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、游离脂肪酸(FFA)、肿瘤坏死因子(TNF-α)、空腹血糖(FBS)和空腹胰岛素(FINS),并计算胰岛素敏感指数(ISI),观察肝脏组织病理变化和炎症活动度计分;应用原位杂交和半定量RT-PCR技术检测各组大鼠肝脏组织抵抗素mRNA的表达及定位。结果模型组肝组织抵抗素mRNA表达较正常组显著性升高,其阳性颗粒主要分布于小叶内及汇管区炎细胞浸润区的巨噬细胞胞浆中。模型组肝组织抵抗素和血清TNF-α水平、肝脏炎症活动度计分成显著性正相关(r=0.873,0.892,P<0.01),但与ISI无相关。结论NASH模型大鼠肝脏抵抗素定位于小叶内及汇管区炎细胞浸润区的巨噬细胞胞浆中,参与NASH的炎症发病机制。
Objective To investigate the expression and localization of resistin mRNA in the rat liver with nonalcoholic steatohepatitis （NASH）. Methods 16 male Wistar rats were randomly divided into standard diet-fed group, and high fat diet-fed group. After 18 weeks,we undertook in situ hybridization and RT-PCR studies to demonstrate the localization and semiquantitation of resistin mRNA in liver tissue. The levels of serum biochemical index including triglyceride,total cholesterol,high density lipoprotein cholesterol,low density lipoprotein cholesterol,free fatty acid,fasting blood glucose,insuline,tumor necrosis factor-α were measured. Results The relative level of resistin mRNA in liver tissue of NASH group was significantly higher compared with controls （2.140,11 vs 0.420.08,P〈0.01）. And its positive signals were observed unique in monocytes/macrophages in the areas of inflammatory infiltration. A positive correlation was found between resistin and histological inflammatory score and plasma TNF-α levels （r=0.892,0.873,P〈0.01）. No correlation was observed between resistin and insulin resistance. Conclusion The intrahepatic resistin locates in monocytes/macrophages in the areas of inflammatory infiltration,and resistin,as a novel modulator of intrahepatic inflammation,may be involved in the pathogenesis or progression of NASH.
出处
《实用肝脏病杂志》
CAS
2008年第4期225-228,共4页
Journal of Practical Hepatology
