摘要
采用木瓜蛋白酶、胰蛋白酶2种酶分步水解技术,对甲鱼蛋白质进行水解。利用均匀设计法研究上述2种酶分步水解甲鱼蛋白质的最佳工艺条件为:原料∶水=1∶3.5,木瓜蛋白酶用量18.0 g.kg-1,温度50℃,pH值6.9,水解时间5.5 h;而胰蛋白酶用量28.0 g.kg-1,温度50℃,pH 7.5,水解时间4 h。所得水解液的氨基氮含量为7906.5 mg.kg-1,水解液游离氨基酸的含量为626.7 mg.(100 mL)-1,其中必需氨基酸的含量为211.35 mg.(100 mL)-1。
The protein of soft-shelled turtle was hydrolyzed with Papain and Trypsin by the enzyme hydrolytic technique. Applied the uniform design and statistical analysis system, the optimum technology conditions were studied： the ratio of protein of soft-shelled turtle to water was 1：3.5（on the weight basis）, enzyme concentration of Papain was 18.0 g·kg^-1, the hydrolysis temperature 50 ℃, pH 6.9, hydrolysis time 5.5 h. Enzyme concentration of Trypsin was 28.0 g·kg^-1, the hydrolysis temperature 50 ℃, pH 7.5, hydrolysis time 4h. The content of amino-N in the hydrolysate was 7906.5 mg·kg^-1, the concentration of free amino acids in the hydroiysate was 626.7 mg· （100 mL）^-1, and the essential amino acids was 211.35 mg· （100 mL）^-1
出处
《化工技术与开发》
CAS
2008年第7期9-12,共4页
Technology & Development of Chemical Industry
关键词
甲鱼
酶水解
均匀设计法
最佳条件
soft-shelled turtle
enzymic hydrolysis
uniform design
optimum condition
