摘要
目的:通过建立裸鼠右肩皮下C6胶质瘤动物模型,研究碘125间质内照射对恶性胶质瘤的治疗作用。方法:Balb/c裸鼠30只。体外培养大鼠C6胶质瘤细胞,接种于裸鼠右肩皮下建立肿瘤模型。肿瘤形成后对照组(15只),注入无碘125材料,治疗组(15只)肿瘤中心注入碘125聚胺酯放射材料,4周时对比两组肿瘤体积、瘤重并处死取病理行组织学检查分析。结果:C6细胞接种后1周,裸鼠皮下均可见肿瘤形成。4周时对照组与治疗组肿瘤体积、瘤重差异明显(P<0.05),病理组织学检查见对照组肿瘤细胞呈典型的恶性星形细胞瘤特征,治疗组肿瘤细胞体积缩小及大量坏死。结论:碘125聚胺酯放射材料可通过间质内照射对C6胶质瘤细胞产生杀伤作用,明显抑制肿瘤生长。
Objective：To develop C6 subcutaneous glioma model in nude mouse and investigate therapeutical effect of ^125I brachytherapy to malignant glioma.Method：C6 glioma cells were cultured in vitro.30 nude mice were inoculated C6 glioma cells in right shoulder subcutaneous tissue to establish tumor models.1 week after inoculation,control group（n=15）tumor were implanted non-radiomaterial;experient group（n=15）tumor were implanted ^125I-Polyamine-ester material.Both group tumor volume and weight was measured to contrast after 4 weeks,histopathologic examination was performed both in control and experient group.Result：Tumor appeared in nude mouse right shoulder about 1 week after C6 cells injection.Tumor volume and weight was significantly different between two group（P〈0.05）after 4 weeks.Typical malignant astrocytic glioma character was observed incontrol group and tumor cell volume diminutus and cellular necrosis was observed in experient group.Conclusion：^125I-Polyamine-ester material can kill and wound C6 malignant glioma cells by brachytherapy,tumor growth was obviously inhibited.
出处
《航空航天医药》
2008年第2期82-84,共3页
Aerospace Medicine
