摘要
目的研究姜黄素在体外诱导角质形成细胞凋亡及其作用机制。方法采用MTT法测定不同浓度的姜黄素对角质形成细胞增殖的影响,流式细胞仪观察细胞凋亡情况,分光光度法检测细胞内半胱天冬蛋白酶3(Caspase-3)及半胱天冬蛋白酶9(Caspase-9)活性的改变情况。结果浓度>5μmol/L的姜黄素处理后细胞早期凋亡率显著升高(P<0.05),细胞内Caspase-3及Caspase-9的活性明显增加(P<0.05),均与姜黄素呈浓度依赖性。结论一定浓度范围的姜黄素可诱导角质形成细胞的凋亡,Caspase-3及Caspase-9活性增加可能为其诱导凋亡的重要机制之一。
Objective To investigate the influence of curcumin on the apoptosis of keratinocyte and its mechanism of action in vitro. Methods The effects of curcumin of consecutive concentrations on the proliferation of HaCaT were observed by MTT assay. Apoptosis was evaluated by flow cytometry. The activity of caspase -3 and caspase -9 was determined by fluorescent analysis. Results After HaCaT were treated with curcumin（ 〉5umol/L）, the percentage of the early apoptotic cells were significantly increased and the levels of Caspase -3 and caspase -9 activity were increased after the treatment . Conclusion Curcumin induces apoptosis of HaCaT cells through activation of caspase -9 and caspase -3.
出处
《中国皮肤性病学杂志》
CAS
北大核心
2008年第6期331-333,共3页
The Chinese Journal of Dermatovenereology
