摘要
目的 探讨在慢性乙肝儿童中HBV基因型分布特性及与临床特征的关系。方法 采用HBV引物特异性基因分型原理，建立巢式PCR方法对404例儿童慢性乙型肝炎患儿的血清HBVDNA基因进行分型。结果 在儿童慢性乙肝中HBVDNA基因型主要为B型（24．5％）和C型（70．5％），其中南方儿童的B型感染率（38．7％）明显高于北方（21．3％，P=O．002）儿童，北方儿童以C型（73．3％）感染为主。B／C型的感染无性别、年龄和母婴传播途径差异。儿童慢性HBV感染者B、C不同的基因型，肝功受损程度（P=O．4796）、血清HBeAg阳性率、HBVDNA病毒载量及肝脏炎症（P=0．209）、纤维化程度（P=0．177）差异均无统计学意义。结论 HBVDNA基因型分布在儿童虽然存在南北方地域的差异。但无母婴传播、病毒复制和肝脏损伤等方面的差异。
Objective To investigate the relation of HBV genotypes to clincal features in children with chronic hepatitis B. Methods The genotypes of serum HBV DNA from 404 children with chronic hepatitis B were determined by PCR using type-specific primers. Results For the 404 children, genotype B in 99 （24.5%）, genotype C in 285 （70.5%）. For the 75 children from south part of China, 29 were of genotype B （38.7%） and 44 of genotype C （58.7%）. For the 329 children from north part of China, 70 were of genotype B （21.3%） and 241 of genotype C （73.3%）. There were significant differences between the children from south part and those from north part of China in genotype B and C （ P = 0.002）. Genotype B and C were not significantly correlated to gener, age and mother-to-fetus transmission. There was no marked difference in liver injury severity （P = 0.4796）, serum HBeAg positivity, HBVDNA level, inflammatory degree of liver tissue （P = 0.209）and liver fibrosis（ P = 0.177） between the children with genotype B and those with genotype C. Conclusion In children with HBV infection, genotype C accouts for 70.5 % and genotype B for 24.5 %. the genotypes are of regional difference in children with HBV infeciton. There are replication and liver pathological change between genotype B and genotype C.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2008年第3期192-194,共3页
Chinese Journal of Experimental and Clinical Virology
